Towards the monitoring of dialysis treatment through absorption and endogenous fluorescence techniques

This study explores the monitoring of uraemic toxins in haemodialysis treatments, beyond urea concentration, in the perspective of reducing complications stemming from the lack of personalized haemodialysis therapies. Absorption and fluorescence techniques were employed to analyse low-middle weight molecules employing laboratory prepared solutions combining p-cresol, bovine albumin and bisphenol-A molecules. These optical techniques offer the advantage of analysing the sample without physical contact, ensuring the potential for implementation in a real-world scenario for continuous treatment monitoring. Real-time monitoring of these toxins in spent dialysate could offer insights into blood concentrations, enhancing dialysis treatment optimization and complication management. The considered absorption spectra fell within the ultraviolet range (240, 400) nm, while fluorescence spectra were obtained at a selected excitation wavelength of 285 nm. Data analysis revealed a correlation between molecule concentrations and signal intensity in both absorption and fluorescence measurements.