Enrichment of strontium and magnesium improves the physical, mechanical and biological properties of bioactive glasses undergoing thermal treatments: New cues for biomedical applications

Bioactive glasses (BGs) have emerged as invaluable resources for bone tissue engineering due to their remarkable properties such as bioactivity, resorbability, cell compatibility, and osteoconductivity. However, these materials exhibit certain limitations when subjected to high temperatures, for their tendency to crystallize, thus leading to diminished bioactivity, reduced mechanical strength, and altered dissolution kinetics. One promising approach to counteract this problem is to reduce the alkaline element content in BGs while simultaneously adding strontium and magnesium. Building on previous studies of Bio_MS, a recently developed experimental formulation, we investigated the contributions of strontium and magnesium to the thermal, mechanical, and biological properties of various bioactive glasses, including commercially available options. Differential thermal analysis, heating microscopy, X-ray diffractometry, environmental scanning electron microscopy, measurement of the Young's modulus, simulated body fluid testing, cytotoxicity tests, cell viability, growth, adhesion and morphology were assessed through an integrated approach and compared for a complete evaluation of BGs, and of doped BGs, also undergoing thermal treatments. The results demonstrated improved thermal, mechanical and biological behaviors of the magnesium-strontium-doped BGs, thus paving the way for the development of BGs with enhanced biomedical perspectives.