High-Resolution Magic Angle Spinning (HR-MAS) Nuclear Magnetic Resonance (NMR) can play an important role among the analytical methods that can be used in metabolomics. It was developed more than 25 years ago,[1] and since then it has been used to analyze cells and biological tissues in many fields: biochemical, biomedical,[2] plants,[3] agriculture,[4] food,[5] etc. Typical problems tackled are information on cellular metabolisms of physiological and pathological processes, response to some kind of stress, differences among cultivars or varieties, metabolism in little living organisms. HR-MAS NMR has become an important platform for metabolomic studies. The technique allows the detection of the metabolic profile directly on intact tissue samples and cells, producing NMR spectra with resolution that can be comparable to that obtained from sample extract solutions, avoiding complex metabolite extraction processes, and preserving tissue and cellular structures. These studies can be based on the analysis of metabolic profiles in their entirety or on the quantification of individual metabolites or metabolite ratios. The basis of the technique, some critical issues and some examples of application in different contexts will be shown. [1] L. L. Cheng, C. L. Lean, A. Bogdanova, S. C. Wright, Jr, J. L. Ackerman, T. J. Brady, L. Garrido, Magnetic resonance in medicine (1996), 36, 653-8 [2] L. L Cheng, NMR in biomedicine (2023), 36, e4684. [3] C. Deborde, A. Moing, L. Roch, D. Jacob, D. Rolin, P. Giraudeau, Progress in Nuclear Magnetic Resonance Spectroscopy (2017), 102-103, 61-97. [4] P. Mazzei, A. Piccolo, Chemical and Biological Technologies in Agriculture (2017), 4, 11/1-11/13. [5] C. Corsaro, N. Cicero, D. Mallamace, S. Vasi, C. Naccari, A. Salvo, S. V. Giofre, G. Dugo, Food Research International (2016), 89(Part_3), 1085-1094.
Tissue metabolomics and HR-MAS NMR / Mucci, Adele. - (2023). (Intervento presentato al convegno Italian metabolomics network - general meeting tenutosi a Genova nel 9-10 novembre 2023).
Tissue metabolomics and HR-MAS NMR
adele mucci
2023
Abstract
High-Resolution Magic Angle Spinning (HR-MAS) Nuclear Magnetic Resonance (NMR) can play an important role among the analytical methods that can be used in metabolomics. It was developed more than 25 years ago,[1] and since then it has been used to analyze cells and biological tissues in many fields: biochemical, biomedical,[2] plants,[3] agriculture,[4] food,[5] etc. Typical problems tackled are information on cellular metabolisms of physiological and pathological processes, response to some kind of stress, differences among cultivars or varieties, metabolism in little living organisms. HR-MAS NMR has become an important platform for metabolomic studies. The technique allows the detection of the metabolic profile directly on intact tissue samples and cells, producing NMR spectra with resolution that can be comparable to that obtained from sample extract solutions, avoiding complex metabolite extraction processes, and preserving tissue and cellular structures. These studies can be based on the analysis of metabolic profiles in their entirety or on the quantification of individual metabolites or metabolite ratios. The basis of the technique, some critical issues and some examples of application in different contexts will be shown. [1] L. L. Cheng, C. L. Lean, A. Bogdanova, S. C. Wright, Jr, J. L. Ackerman, T. J. Brady, L. Garrido, Magnetic resonance in medicine (1996), 36, 653-8 [2] L. L Cheng, NMR in biomedicine (2023), 36, e4684. [3] C. Deborde, A. Moing, L. Roch, D. Jacob, D. Rolin, P. Giraudeau, Progress in Nuclear Magnetic Resonance Spectroscopy (2017), 102-103, 61-97. [4] P. Mazzei, A. Piccolo, Chemical and Biological Technologies in Agriculture (2017), 4, 11/1-11/13. [5] C. Corsaro, N. Cicero, D. Mallamace, S. Vasi, C. Naccari, A. Salvo, S. V. Giofre, G. Dugo, Food Research International (2016), 89(Part_3), 1085-1094.File | Dimensione | Formato | |
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