Matrix metalloproteinase-9 (MMP-9) is involved in tissue remodeling and in skin wound healing. The present study focuses on the MMP-9 expression in epidermal wound healing within 1 h after injury, to test whether MMP-9 can be used to estimate the time of injury in forensic practice. A sample consisting of 5 individuals undergoing surgery was analyzed. With the consent of the patients, sections of skin were removed from the surgical wound at predefined time intervals. For each subject, 8 sections were taken, one for each time interval defined at 0 ‘- 1’ − 3 ‘- 5’ − 10 ‘- 15’ − 30 ‘- 60’ minutes. The specimens were immunostained with MMP-9, and the number of positively stained cells was examined. The number of positively stained cells showed an increasing trend as a function of time. Less than 30 positively stained cells were found in all cases within 3 min. At the post-infliction time of 5 min, the number of positively stained cells exceeded 30 in 3 out of 5 cases. The number of MMP-positive cells exceeded 40 in all cases in over 10 min. In the light of these results, the count of MMP-9 positive cells might be a useful marker in the wound-age estimation within 1 h in forensic setting. More research is required to collect more samples and to compare samples from the hyperacute phase with those from several days after injury.

Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study / Cecchi, R.; Tomoya, I.; Camatti, J.; Mizuho, N.; Yuko, I.; Toshikazu, K.. - In: INTERNATIONAL JOURNAL OF LEGAL MEDICINE. - ISSN 0937-9827. - 138:5(2024), pp. 1985-1990. [10.1007/s00414-024-03243-x]

Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study

Cecchi R.;Camatti J.;
2024

Abstract

Matrix metalloproteinase-9 (MMP-9) is involved in tissue remodeling and in skin wound healing. The present study focuses on the MMP-9 expression in epidermal wound healing within 1 h after injury, to test whether MMP-9 can be used to estimate the time of injury in forensic practice. A sample consisting of 5 individuals undergoing surgery was analyzed. With the consent of the patients, sections of skin were removed from the surgical wound at predefined time intervals. For each subject, 8 sections were taken, one for each time interval defined at 0 ‘- 1’ − 3 ‘- 5’ − 10 ‘- 15’ − 30 ‘- 60’ minutes. The specimens were immunostained with MMP-9, and the number of positively stained cells was examined. The number of positively stained cells showed an increasing trend as a function of time. Less than 30 positively stained cells were found in all cases within 3 min. At the post-infliction time of 5 min, the number of positively stained cells exceeded 30 in 3 out of 5 cases. The number of MMP-positive cells exceeded 40 in all cases in over 10 min. In the light of these results, the count of MMP-9 positive cells might be a useful marker in the wound-age estimation within 1 h in forensic setting. More research is required to collect more samples and to compare samples from the hyperacute phase with those from several days after injury.
2024
138
5
1985
1990
Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study / Cecchi, R.; Tomoya, I.; Camatti, J.; Mizuho, N.; Yuko, I.; Toshikazu, K.. - In: INTERNATIONAL JOURNAL OF LEGAL MEDICINE. - ISSN 0937-9827. - 138:5(2024), pp. 1985-1990. [10.1007/s00414-024-03243-x]
Cecchi, R.; Tomoya, I.; Camatti, J.; Mizuho, N.; Yuko, I.; Toshikazu, K.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1347349
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