Policosanols (PCs) are bioactive compounds extracted from different natural waxes. In this work, the purification, characterization and assessment of the antioxidant and anti-inflammatory activity was carried out on PCs from an innovative source, i.e. a waxy material from supercriticalfluid extraction (SFE) of non -psychoactive Cannabis sativa L. (hemp) inflorescences. Starting from this material, PCs were obtained by microwave -assisted trans -esterification and hydrolysis, followed by preparative liquid chromatography under normal phase conditions. The purified product was characterized using high-performance liquid chromatography (HPLC) with an evaporative light scattering detector (ELSD). In vitro cell -free and cell -based antioxidant and antiinflammatory assays were then performed to assess their bioactivity.
Extraction, purification and in vitro assessment of the antioxidant and anti-inflammatory activity of policosanols from non-psychoactive Cannabis sativa L / Caroli, Clarissa; Baron, Giovanna; Cappellucci, Giorgio; Brighenti, Virginia; Della Vedova, Larissa; Fraulini, Francesca; Oliaro-Bosso, Simonetta; Alessandrini, Andrea; Zambon, Alfonso; Lusvardi, Gigliola; Aldini, Giancarlo; Biagi, Marco; Corsi, Lorenzo; Pellati, Federica. - In: HELIYON. - ISSN 2405-8440. - 10:9(2024), pp. 1-10. [10.1016/j.heliyon.2024.e30291]
Extraction, purification and in vitro assessment of the antioxidant and anti-inflammatory activity of policosanols from non-psychoactive Cannabis sativa L
Caroli, Clarissa;Brighenti, Virginia;Fraulini, Francesca;Alessandrini, AndreaMembro del Collaboration Group
;Zambon, Alfonso;Lusvardi, Gigliola;Corsi, Lorenzo;Pellati, Federica
2024
Abstract
Policosanols (PCs) are bioactive compounds extracted from different natural waxes. In this work, the purification, characterization and assessment of the antioxidant and anti-inflammatory activity was carried out on PCs from an innovative source, i.e. a waxy material from supercriticalfluid extraction (SFE) of non -psychoactive Cannabis sativa L. (hemp) inflorescences. Starting from this material, PCs were obtained by microwave -assisted trans -esterification and hydrolysis, followed by preparative liquid chromatography under normal phase conditions. The purified product was characterized using high-performance liquid chromatography (HPLC) with an evaporative light scattering detector (ELSD). In vitro cell -free and cell -based antioxidant and antiinflammatory assays were then performed to assess their bioactivity.
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