The technology for producing microelectrode arrays (MEAs) has been developing since the 1970s and extracellular electrophysiological recordings have become well established in neuroscience, drug screening and cardiology. MEAs allow monitoring of long-term spiking activity of large ensembles of excitable cells noninvasively with high temporal resolution and mapping its spatial features. However, their inability to register subthreshold potentials, such as intrinsic membrane oscillations and synaptic potentials, has inspired a number of laboratories to search for alternatives to bypass the restrictions and/or increase the sensitivity of microelectrodes. In this study, we present the fabrication and in vitro experimental validation of arrays of PEDOT:PSS-coated 3D ultramicroelectrodes, with the best-reported combination of small size and low electrochemical impedance. We observed that this type of microelectrode does not alter neuronal network biological properties, improves the signal quality of extracellular recordings and exhibits higher selectivity toward single unit recordings. With fabrication processes simpler than those reported in the literature for similar electrodes, our technology is a promising tool for study of neuronal networks. © Copyright © 2020 Jones, Moskalyuk, Barthold, Gutöhrlein, Heusel, Schröppel, Samba and Giugliano.

Low-Impedance 3D PEDOT:PSS Ultramicroelectrodes / Jones, Pd; Moskalyuk, A; Barthold, C; Gutöhrlein, K; Heusel, G; Schröppel, B; Samba, R; Giugliano, M.. - In: FRONTIERS IN NEUROSCIENCE. - ISSN 1662-4548. - 14:(2020), pp. 1-12. [10.3389/fnins.2020.00405]

Low-Impedance 3D PEDOT:PSS Ultramicroelectrodes

Giugliano M.
2020

Abstract

The technology for producing microelectrode arrays (MEAs) has been developing since the 1970s and extracellular electrophysiological recordings have become well established in neuroscience, drug screening and cardiology. MEAs allow monitoring of long-term spiking activity of large ensembles of excitable cells noninvasively with high temporal resolution and mapping its spatial features. However, their inability to register subthreshold potentials, such as intrinsic membrane oscillations and synaptic potentials, has inspired a number of laboratories to search for alternatives to bypass the restrictions and/or increase the sensitivity of microelectrodes. In this study, we present the fabrication and in vitro experimental validation of arrays of PEDOT:PSS-coated 3D ultramicroelectrodes, with the best-reported combination of small size and low electrochemical impedance. We observed that this type of microelectrode does not alter neuronal network biological properties, improves the signal quality of extracellular recordings and exhibits higher selectivity toward single unit recordings. With fabrication processes simpler than those reported in the literature for similar electrodes, our technology is a promising tool for study of neuronal networks. © Copyright © 2020 Jones, Moskalyuk, Barthold, Gutöhrlein, Heusel, Schröppel, Samba and Giugliano.
2020
14
1
12
Low-Impedance 3D PEDOT:PSS Ultramicroelectrodes / Jones, Pd; Moskalyuk, A; Barthold, C; Gutöhrlein, K; Heusel, G; Schröppel, B; Samba, R; Giugliano, M.. - In: FRONTIERS IN NEUROSCIENCE. - ISSN 1662-4548. - 14:(2020), pp. 1-12. [10.3389/fnins.2020.00405]
Jones, Pd; Moskalyuk, A; Barthold, C; Gutöhrlein, K; Heusel, G; Schröppel, B; Samba, R; Giugliano, M.
File in questo prodotto:
File Dimensione Formato  
fnins-14-00405.pdf

Open access

Tipologia: Versione pubblicata dall'editore
Dimensione 4.45 MB
Formato Adobe PDF
4.45 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1333803
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 23
social impact