A pillar of wine authenticity is the variety/ies used. Ampelographic descriptors and SSR markers, included in several national and international databases, are extensively used for varietal identification purposes. Recently, SNP markers have been proposed as useful for grape varietal identification and traceability. Our study has been directed toward the development of a molecular toolbox able to track grape varieties from the nursery to the must. Two complementary approaches were developed, exploiting SNP markers with two different technologies, i.e., a high-throughput platform for varietal identification and a digital PCR system for varietal quantification. As proof-of-concept, the toolbox was successfully applied to the identification and quantification of the “Glera” variety along the Prosecco wine production chain. The assays developed found their limits in commercial, aged wines.

A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera” / Carrara, I.; Terzi, V.; Ghizzoni, R.; Delbono, S.; Tumino, G.; Crespan, M.; Gardiman, M.; Francia, E.; Morcia, C.. - In: FOODS. - ISSN 2304-8158. - 12:16(2023), pp. 3091-3108. [10.3390/foods12163091]

A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”

Terzi V.
;
Tumino G.;Francia E.;
2023

Abstract

A pillar of wine authenticity is the variety/ies used. Ampelographic descriptors and SSR markers, included in several national and international databases, are extensively used for varietal identification purposes. Recently, SNP markers have been proposed as useful for grape varietal identification and traceability. Our study has been directed toward the development of a molecular toolbox able to track grape varieties from the nursery to the must. Two complementary approaches were developed, exploiting SNP markers with two different technologies, i.e., a high-throughput platform for varietal identification and a digital PCR system for varietal quantification. As proof-of-concept, the toolbox was successfully applied to the identification and quantification of the “Glera” variety along the Prosecco wine production chain. The assays developed found their limits in commercial, aged wines.
2023
12
16
3091
3108
A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera” / Carrara, I.; Terzi, V.; Ghizzoni, R.; Delbono, S.; Tumino, G.; Crespan, M.; Gardiman, M.; Francia, E.; Morcia, C.. - In: FOODS. - ISSN 2304-8158. - 12:16(2023), pp. 3091-3108. [10.3390/foods12163091]
Carrara, I.; Terzi, V.; Ghizzoni, R.; Delbono, S.; Tumino, G.; Crespan, M.; Gardiman, M.; Francia, E.; Morcia, C.
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