In a previous immunohistochemical (IHC) study, we documented the reaction of lung tissue vessels to hypoxia through the immunodetection of HIF1-α protein, a key regulator of cellular response to hypoxic conditions. Findings showing that asphyxia deaths are associated with an increase in the number of mast cell (MC)-derived tryptase enzymes in the blood suggests that HIF1-α production may be correlated with MC activation in hypoxic conditions. This hypothesis prompted us to investigate the possible role of pulmonary MC in acute asphyxia deaths. Lung of 47 medico-legal autopsy cases (35 asphyxia/hypoxia deaths, 11 controls, and 1 anaphylactic death) were processed by IHC analysis using anti-CD117 (c-Kit) antibody to investigate peri-airway and perivascular MC together with their counts and features. Results showed a significant increase in peri-vascular c-kit+ MC in some asphyxia deaths, such as hanging, strangulation, and aspiration deaths. A strong activation of MC in peri-airway and peri-vascular areas was also observed in lung samples from the anaphylaxis case, which was used as a positive control. Our study points to the potential role of MC in hypoxia and suggests that an evaluation of MC in the lungs may be a useful parameter when forensic pathologists are required to make a differential diagnosis between acute asphyxia deaths and other kinds of death.

Are mast cells implicated in asphyxia? / Cecchi, Rossana; C., Ciallella; L., Cipolloni; C., Sestili; C., Terranova; B., Muciaccia. - In: INTERNATIONAL JOURNAL OF LEGAL MEDICINE. - ISSN 1437-1596. - 130:1(2016), pp. 153-161. [10.1007/s00414-015-1211-5]

Are mast cells implicated in asphyxia?

CECCHI, Rossana;
2016

Abstract

In a previous immunohistochemical (IHC) study, we documented the reaction of lung tissue vessels to hypoxia through the immunodetection of HIF1-α protein, a key regulator of cellular response to hypoxic conditions. Findings showing that asphyxia deaths are associated with an increase in the number of mast cell (MC)-derived tryptase enzymes in the blood suggests that HIF1-α production may be correlated with MC activation in hypoxic conditions. This hypothesis prompted us to investigate the possible role of pulmonary MC in acute asphyxia deaths. Lung of 47 medico-legal autopsy cases (35 asphyxia/hypoxia deaths, 11 controls, and 1 anaphylactic death) were processed by IHC analysis using anti-CD117 (c-Kit) antibody to investigate peri-airway and perivascular MC together with their counts and features. Results showed a significant increase in peri-vascular c-kit+ MC in some asphyxia deaths, such as hanging, strangulation, and aspiration deaths. A strong activation of MC in peri-airway and peri-vascular areas was also observed in lung samples from the anaphylaxis case, which was used as a positive control. Our study points to the potential role of MC in hypoxia and suggests that an evaluation of MC in the lungs may be a useful parameter when forensic pathologists are required to make a differential diagnosis between acute asphyxia deaths and other kinds of death.
2016
130
1
153
161
Are mast cells implicated in asphyxia? / Cecchi, Rossana; C., Ciallella; L., Cipolloni; C., Sestili; C., Terranova; B., Muciaccia. - In: INTERNATIONAL JOURNAL OF LEGAL MEDICINE. - ISSN 1437-1596. - 130:1(2016), pp. 153-161. [10.1007/s00414-015-1211-5]
Cecchi, Rossana; C., Ciallella; L., Cipolloni; C., Sestili; C., Terranova; B., Muciaccia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1318035
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