Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. We autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
[Moist autoclaving. A simplified method for antigen unmasking] / A., Bankfalvi; K., Riehemann; D., Oeffner; Cecchi, Rossana; J. M., Morgan; J., Piffko; W., Boecker; B., Jasani; K. W., Schmid. - In: PATHOLOGE. - ISSN 0172-8113. - 15:6(1994), pp. 345-349.
[Moist autoclaving. A simplified method for antigen unmasking]
CECCHI, Rossana;
1994
Abstract
Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. We autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
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