Malignant gliomas, the most frequent primary brain tumors, are characterized by a dismal prognosis. Reliable biomarkers complementary to neuroradiology in the differential diagnosis of gliomas and monitoring for post-surgical progression are unmet needs. Altered expression of several microRNAs in tumour tissues from patients with gliomas compared to normal brain tissue have been described, thus supporting the rationale of using microRNA-based biomarkers. Although different circulating microRNAs were proposed in association with gliomas, they have not been introduced into clinical practice so far. Blood samples were collected from patients with high and low grade gliomas, both before and after surgical resection, and the expression of miR-21, miR-222 and miR-124-3p was measured in exosomes isolated from serum. The expression levels of miR-21, miR-222 and miR-124-3p in serum exosomes of patients with high grade gliomas were significantly higher than those of low grade gliomas and healthy controls and were sharply decreased in samples obtained after surgery. The analysis of miR-21, miR-222 and miR-124-3p in serum exosomes of patients affected by gliomas can provide a minimally invasive and innovative tool to help the differential diagnosis of gliomas at their onset in the brain and predict glioma grading and non glial metastases before surgery.

A microRNA signature from serum exosomes of patients with glioma as complementary diagnostic biomarker / Santangelo, Alessandra; Imbruce', Pietro; Gardenghi, Beatrice; Belli, Laura; Agushi, Rina; Tamanini, Anna; Munari, Silvia; Bossi, Alessandra Maria; Scambi, Ilaria; Benati, Donatella; Mariotti, Raffaella; DI GENNARO, Gianfranco; Sbarbati, Andrea; Eccher, Albino; Ricciardi, GIUSEPPE KENNETH; Ciceri, Elisa Maria; Sala, Francesco; Pinna, Giampietro; Lippi, Giuseppe; Cabrini, Giulio; Dechecchi, Maria Cristina. - In: JOURNAL OF NEURO-ONCOLOGY. - ISSN 0167-594X. - 136:1(2018), pp. 51-62. [10.1007/s11060-017-2639-x]

A microRNA signature from serum exosomes of patients with glioma as complementary diagnostic biomarker

ECCHER, Albino;
2018

Abstract

Malignant gliomas, the most frequent primary brain tumors, are characterized by a dismal prognosis. Reliable biomarkers complementary to neuroradiology in the differential diagnosis of gliomas and monitoring for post-surgical progression are unmet needs. Altered expression of several microRNAs in tumour tissues from patients with gliomas compared to normal brain tissue have been described, thus supporting the rationale of using microRNA-based biomarkers. Although different circulating microRNAs were proposed in association with gliomas, they have not been introduced into clinical practice so far. Blood samples were collected from patients with high and low grade gliomas, both before and after surgical resection, and the expression of miR-21, miR-222 and miR-124-3p was measured in exosomes isolated from serum. The expression levels of miR-21, miR-222 and miR-124-3p in serum exosomes of patients with high grade gliomas were significantly higher than those of low grade gliomas and healthy controls and were sharply decreased in samples obtained after surgery. The analysis of miR-21, miR-222 and miR-124-3p in serum exosomes of patients affected by gliomas can provide a minimally invasive and innovative tool to help the differential diagnosis of gliomas at their onset in the brain and predict glioma grading and non glial metastases before surgery.
2018
136
1
51
62
A microRNA signature from serum exosomes of patients with glioma as complementary diagnostic biomarker / Santangelo, Alessandra; Imbruce', Pietro; Gardenghi, Beatrice; Belli, Laura; Agushi, Rina; Tamanini, Anna; Munari, Silvia; Bossi, Alessandra Maria; Scambi, Ilaria; Benati, Donatella; Mariotti, Raffaella; DI GENNARO, Gianfranco; Sbarbati, Andrea; Eccher, Albino; Ricciardi, GIUSEPPE KENNETH; Ciceri, Elisa Maria; Sala, Francesco; Pinna, Giampietro; Lippi, Giuseppe; Cabrini, Giulio; Dechecchi, Maria Cristina. - In: JOURNAL OF NEURO-ONCOLOGY. - ISSN 0167-594X. - 136:1(2018), pp. 51-62. [10.1007/s11060-017-2639-x]
Santangelo, Alessandra; Imbruce', Pietro; Gardenghi, Beatrice; Belli, Laura; Agushi, Rina; Tamanini, Anna; Munari, Silvia; Bossi, Alessandra Maria; Scambi, Ilaria; Benati, Donatella; Mariotti, Raffaella; DI GENNARO, Gianfranco; Sbarbati, Andrea; Eccher, Albino; Ricciardi, GIUSEPPE KENNETH; Ciceri, Elisa Maria; Sala, Francesco; Pinna, Giampietro; Lippi, Giuseppe; Cabrini, Giulio; Dechecchi, Maria Cristina
File in questo prodotto:
File Dimensione Formato  
Santangelo2018_Article_AMicroRNASignatureFromSerumExo (1).pdf

Accesso riservato

Dimensione 1.52 MB
Formato Adobe PDF
1.52 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1317422
Citazioni
  • ???jsp.display-item.citation.pmc??? 76
  • Scopus 119
  • ???jsp.display-item.citation.isi??? 107
social impact