Intra-operative blood cell salvage (IOCS) is mainly avoided in onco surgery due to the suspicion that it could increase metastasis’ risk. We simulated IOCS followed by leucodepletion: HCT116 (human colorectal cancer) cells were inoculated into packed red blood cells units, and their distribution was evaluated, step-by-step, by flow cytometry and immunohistochemistry. Most of HCT116 cells were lost during washing, and almost completely removed after filtration. IOCS plus leucodepletion could be of great advantage for oncological patients, where allogenic blood transfusion could influence tumour progression.
Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model / Marraccini, C.; Merolle, L.; Berni, P.; Boito, K.; Tamagnini, I.; Kuhn, E.; Ragazzi, M.; Baricchi, R.; Pertinhez, T. A.. - In: VOX SANGUINIS. - ISSN 0042-9007. - 112:8(2017), pp. 803-805. [10.1111/vox.12565]
Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model
Marraccini C.;Merolle L.;Ragazzi M.;
2017
Abstract
Intra-operative blood cell salvage (IOCS) is mainly avoided in onco surgery due to the suspicion that it could increase metastasis’ risk. We simulated IOCS followed by leucodepletion: HCT116 (human colorectal cancer) cells were inoculated into packed red blood cells units, and their distribution was evaluated, step-by-step, by flow cytometry and immunohistochemistry. Most of HCT116 cells were lost during washing, and almost completely removed after filtration. IOCS plus leucodepletion could be of great advantage for oncological patients, where allogenic blood transfusion could influence tumour progression.
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