Among perinatal stem cells of the umbilical cord, human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) are of great interest for cell-based therapy approaches in regenerative medicine, showing some advantages over other MSCs. In fact, hWJ-MSCs, placed between embryonic and adult MSCs, are not tumorigenic and are harvested with few ethical concerns. Furthermore, these cells can be easily cultured in vitro, maintaining both stem properties and a high proliferative rate for several passages, as well as trilineage capacity of differentiation. Recently, it has been demonstrated that cytoskeletal organization influences stem cell biology. Among molecules able to modulate its dynamics, Cytochalasin B (CB), a cyto-permeable mycotoxin, influences actin microfilament polymerization, thus affecting several cell properties, such as the ability of MSCs to differentiate towards a specific commitment. Here, we investigated for the first time the effects of a 24 h-treatment with CB at different concentrations (0.1-3 mu M) on hWJ-MSCs. CB influenced the cytoskeletal organization in a dose-dependent manner, inducing changes in cell number, proliferation, shape, and nanomechanical properties, thus promoting the osteogenic commitment of hWJ-MSCs, as confirmed by the expression analysis of osteogenic/autophagy markers.

Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton's Jelly Mesenchymal Stem Cells / Pampanella, L; Abruzzo, Pm; Tassinari, R; Alessandrini, A; Petrocelli, G; Ragazzini, G; Cavallini, C; Pizzuti, V; Collura, N; Canaider, S; Facchin, F; Ventura, C. - In: PHARMACEUTICALS. - ISSN 1424-8247. - 16:2(2023), pp. 289-306. [10.3390/ph16020289]

Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton's Jelly Mesenchymal Stem Cells

Alessandrini, A
Membro del Collaboration Group
;
Ragazzini, G
Membro del Collaboration Group
;
2023

Abstract

Among perinatal stem cells of the umbilical cord, human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) are of great interest for cell-based therapy approaches in regenerative medicine, showing some advantages over other MSCs. In fact, hWJ-MSCs, placed between embryonic and adult MSCs, are not tumorigenic and are harvested with few ethical concerns. Furthermore, these cells can be easily cultured in vitro, maintaining both stem properties and a high proliferative rate for several passages, as well as trilineage capacity of differentiation. Recently, it has been demonstrated that cytoskeletal organization influences stem cell biology. Among molecules able to modulate its dynamics, Cytochalasin B (CB), a cyto-permeable mycotoxin, influences actin microfilament polymerization, thus affecting several cell properties, such as the ability of MSCs to differentiate towards a specific commitment. Here, we investigated for the first time the effects of a 24 h-treatment with CB at different concentrations (0.1-3 mu M) on hWJ-MSCs. CB influenced the cytoskeletal organization in a dose-dependent manner, inducing changes in cell number, proliferation, shape, and nanomechanical properties, thus promoting the osteogenic commitment of hWJ-MSCs, as confirmed by the expression analysis of osteogenic/autophagy markers.
2023
16
2
289
306
Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton's Jelly Mesenchymal Stem Cells / Pampanella, L; Abruzzo, Pm; Tassinari, R; Alessandrini, A; Petrocelli, G; Ragazzini, G; Cavallini, C; Pizzuti, V; Collura, N; Canaider, S; Facchin, F; Ventura, C. - In: PHARMACEUTICALS. - ISSN 1424-8247. - 16:2(2023), pp. 289-306. [10.3390/ph16020289]
Pampanella, L; Abruzzo, Pm; Tassinari, R; Alessandrini, A; Petrocelli, G; Ragazzini, G; Cavallini, C; Pizzuti, V; Collura, N; Canaider, S; Facchin, F; Ventura, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1300846
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