Abstract MicroRNAs are small single-stranded molecules that have emerged as important genomic regulators in different pathways. Different studies have shown that they are implicated in the metabolism and glucose homeostasis, and therefore, they could also be involved in the pathogenesis of metabolic disorders such as type 2 diabetes (T2DM). The aim of this study was to verify whether genetic variations in candidate microRNA (miRNA or miR) genes could contribute to T2DM susceptibility. We have selected 13 miRNAs as candidate loci according to literature data and to a computational analysis. MicroRNA genes were analyzed by direct sequencing in a cohort of 163 Italian T2DM patients and 185 healthy controls. We identified 6 novel variants never described before and 9 SNPs already described in databases. Five newly identified variants were found only in the cases group. We performed a case/control association study to test the associations of particular alleles/genotypes of identified SNPs with the disease. Two polymorphisms were associated with T2DM susceptibility: in particular, the G allele of rs895819 in hsa-mir-27a has shown a significantly protective effect (OR = 0.58 and P = 0.008), while the G allele of rs531564 in hsa-mir-124a appears to be a risk allele (OR = 2.15, P = 0.008). This is the first report indicating that genetic polymorphisms in miRNA regions could contribute to T2DM susceptibility.

MicroRNA genetic variations: association with type 2 diabetes / Ciccacci, Cinzia; Di Fusco, Davide; Cacciotti, Laura; Morganti, Roberto; D'Amato, Cinzia; Greco, Carla; Rufini, Sara; Novelli, Giuseppe; Sangiuolo, Federica; Spallone, Vincenza; Borgiani, Paola. - In: ACTA DIABETOLOGICA. - ISSN 0940-5429. - 50:6(2013), pp. 867-872. [10.1007/s00592-013-0469-7]

MicroRNA genetic variations: association with type 2 diabetes.

Greco Carla;Spallone Vincenza;
2013

Abstract

Abstract MicroRNAs are small single-stranded molecules that have emerged as important genomic regulators in different pathways. Different studies have shown that they are implicated in the metabolism and glucose homeostasis, and therefore, they could also be involved in the pathogenesis of metabolic disorders such as type 2 diabetes (T2DM). The aim of this study was to verify whether genetic variations in candidate microRNA (miRNA or miR) genes could contribute to T2DM susceptibility. We have selected 13 miRNAs as candidate loci according to literature data and to a computational analysis. MicroRNA genes were analyzed by direct sequencing in a cohort of 163 Italian T2DM patients and 185 healthy controls. We identified 6 novel variants never described before and 9 SNPs already described in databases. Five newly identified variants were found only in the cases group. We performed a case/control association study to test the associations of particular alleles/genotypes of identified SNPs with the disease. Two polymorphisms were associated with T2DM susceptibility: in particular, the G allele of rs895819 in hsa-mir-27a has shown a significantly protective effect (OR = 0.58 and P = 0.008), while the G allele of rs531564 in hsa-mir-124a appears to be a risk allele (OR = 2.15, P = 0.008). This is the first report indicating that genetic polymorphisms in miRNA regions could contribute to T2DM susceptibility.
2013
50
6
867
872
MicroRNA genetic variations: association with type 2 diabetes / Ciccacci, Cinzia; Di Fusco, Davide; Cacciotti, Laura; Morganti, Roberto; D'Amato, Cinzia; Greco, Carla; Rufini, Sara; Novelli, Giuseppe; Sangiuolo, Federica; Spallone, Vincenza; Borgiani, Paola. - In: ACTA DIABETOLOGICA. - ISSN 0940-5429. - 50:6(2013), pp. 867-872. [10.1007/s00592-013-0469-7]
Ciccacci, Cinzia; Di Fusco, Davide; Cacciotti, Laura; Morganti, Roberto; D'Amato, Cinzia; Greco, Carla; Rufini, Sara; Novelli, Giuseppe; Sangiuolo, Federica; Spallone, Vincenza; Borgiani, Paola
File in questo prodotto:
File Dimensione Formato  
2_2013_MicroRNA_ActaDiabetol_Ciccacci C.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 182.82 kB
Formato Adobe PDF
182.82 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1299891
Citazioni
  • ???jsp.display-item.citation.pmc??? 30
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 58
social impact