Seeds may harbour plant pathogens and, therefore, may be regarded as a possible pathway for their dissemination along trade routes worldwide. Heat treatment appears to be a proper seed sanitation method to comply with the requirement of phytosanitary measures. Carrot seeds have been supposed as a pathway for the dissemination of Candidatus Liberibacter solanacearum, although vertical pathogen transmission is still under debate: nonetheless, such seeds may be conveniently heat treated as a sanitation method. We used a dry thermal treatment (50 ± 1 °C for 72 h) to sanitize seeds from Ca. Liberibacter solanacearum and, since this bacterium is not cultivable, we additionally implemented and evaluated a viability qPCR protocol able to detect only viable cells, therefore confirming seed sanitation. The population of the pathogen was estimated through a plasmid calibration curve. The infection of Ca. Liberibacter solanacearum was quantified in two positive samples at approx. 5 × 106 cells gram −1 of seed. Seed sample tested with monoazides (EMA and PMA) qPCR clearly indicated that seed-associated bacteria lost their viability during the heat treatment, thus confirming seed sanitation. Finally, such heat treatment did not affect seed viability and seed quality: conversely, a remarkable reduction of seed saprophytes was observed, together with a better vigour germination performance.

A viability qPCR protocol to assess the efficacy of a heat treatment to sanitize carrot seeds from Candidatus Liberibacter solanacearum / Ben Othmen, Soukaina; Conti Nibali, Gianmarco; Cassanelli, Stefano; Giovanardi, Davide; Stefani, Emilio. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 1573-8469. - 166:1(2023), pp. 77-90. [10.1007/s10658-023-02646-7]

A viability qPCR protocol to assess the efficacy of a heat treatment to sanitize carrot seeds from Candidatus Liberibacter solanacearum

Stefano Cassanelli;Davide Giovanardi
;
Emilio Stefani
2023

Abstract

Seeds may harbour plant pathogens and, therefore, may be regarded as a possible pathway for their dissemination along trade routes worldwide. Heat treatment appears to be a proper seed sanitation method to comply with the requirement of phytosanitary measures. Carrot seeds have been supposed as a pathway for the dissemination of Candidatus Liberibacter solanacearum, although vertical pathogen transmission is still under debate: nonetheless, such seeds may be conveniently heat treated as a sanitation method. We used a dry thermal treatment (50 ± 1 °C for 72 h) to sanitize seeds from Ca. Liberibacter solanacearum and, since this bacterium is not cultivable, we additionally implemented and evaluated a viability qPCR protocol able to detect only viable cells, therefore confirming seed sanitation. The population of the pathogen was estimated through a plasmid calibration curve. The infection of Ca. Liberibacter solanacearum was quantified in two positive samples at approx. 5 × 106 cells gram −1 of seed. Seed sample tested with monoazides (EMA and PMA) qPCR clearly indicated that seed-associated bacteria lost their viability during the heat treatment, thus confirming seed sanitation. Finally, such heat treatment did not affect seed viability and seed quality: conversely, a remarkable reduction of seed saprophytes was observed, together with a better vigour germination performance.
2023
feb-2023
166
1
77
90
A viability qPCR protocol to assess the efficacy of a heat treatment to sanitize carrot seeds from Candidatus Liberibacter solanacearum / Ben Othmen, Soukaina; Conti Nibali, Gianmarco; Cassanelli, Stefano; Giovanardi, Davide; Stefani, Emilio. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 1573-8469. - 166:1(2023), pp. 77-90. [10.1007/s10658-023-02646-7]
Ben Othmen, Soukaina; Conti Nibali, Gianmarco; Cassanelli, Stefano; Giovanardi, Davide; Stefani, Emilio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1296665
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