In this work, we realized a printed immunosensor based on a competitive enzyme-linked immunosorbent assay (ELISA) to allow histamine detection. We investigated the effect of modifying the immunosensor electrodes with spray-deposited poly(3,4-ethyldioxythiophene)poly(styrenesulfunate) (PEDOT:PSS) and single walled carbon nanotube (SWCNT) films. Cyclic voltammetry (CV) was used to characterize the sensor performance in terms of electron transfer kinetics and to prove an enhancement of the electrode active surface area up to 29.1%, due to the use of the additional PEDOT:PSS/SWCNT film. The calibration curve of the so-realized immunosensor (obtained by chronoamperometry measurements) shows a linear detection range of histamine from 0.05 to 50 ng/ml, with 95.03% coefficient of determination.
A PEDOT: PSS/SWCNT-Coated Screen Printed Immunosensor for Histamine Detection in Food Samples / Bajramshahe, Shkodra; Ali, Douaki; Biresaw, D Abera; Pietro, Ibba; Enrico, Avancini; Cantarella, G; Luisa, Petti; Paolo, Lugli. - 2020-:(2020). ( 52nd IEEE International Symposium on Circuits and Systems, ISCAS 2020 Virtuale 2020) [10.1109/ISCAS45731.2020.9181296].
A PEDOT: PSS/SWCNT-Coated Screen Printed Immunosensor for Histamine Detection in Food Samples
Cantarella G;
2020
Abstract
In this work, we realized a printed immunosensor based on a competitive enzyme-linked immunosorbent assay (ELISA) to allow histamine detection. We investigated the effect of modifying the immunosensor electrodes with spray-deposited poly(3,4-ethyldioxythiophene)poly(styrenesulfunate) (PEDOT:PSS) and single walled carbon nanotube (SWCNT) films. Cyclic voltammetry (CV) was used to characterize the sensor performance in terms of electron transfer kinetics and to prove an enhancement of the electrode active surface area up to 29.1%, due to the use of the additional PEDOT:PSS/SWCNT film. The calibration curve of the so-realized immunosensor (obtained by chronoamperometry measurements) shows a linear detection range of histamine from 0.05 to 50 ng/ml, with 95.03% coefficient of determination.
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