CHEMICAL CROSSLINKING - A USEFUL TOOL FOR EVALUATIONS OF STEROID-RECEPTOR STRUCTURES AND THEIR FUNCTIONAL-STATES IN INTACT-CELLS

Rossini, Gian Paolo; Masci, G.

doi:10.1016/0022-4731(89)90109-X

A procedure of chemical crosslinking of intact cells with glutaraldehyde was employed to contribute to the understanding of glucocorticoid receptor structures and their functional states in vivo. Under optimal experimental conditions, glucocorticoid binding sites were found almost equally distributed between cytosolic and nuclear fractions of crosslinked cells. Sedimentation properties of crosslinked receptor complexes in cytosolic and nuclear extracts revealed that these entities were oligomers, which heterogeneously sedimented between 11 and 4S in the presence of 0.3 M NaCl. By anion exchange chromatography, we could establish that these receptor complex oligomers behaved as untransformed forms.

CHEMICAL CROSSLINKING - A USEFUL TOOL FOR EVALUATIONS OF STEROID-RECEPTOR STRUCTURES AND THEIR FUNCTIONAL-STATES IN INTACT-CELLS / Rossini, Gian Paolo; Masci, G.. - In: JOURNAL OF STEROID BIOCHEMISTRY. - ISSN 0022-4731. - STAMPA. - 34(1989), pp. 363-367. [10.1016/0022-4731(89)90109-X]

Data di pubblicazione:	1989
Titolo:	CHEMICAL CROSSLINKING - A USEFUL TOOL FOR EVALUATIONS OF STEROID-RECEPTOR STRUCTURES AND THEIR FUNCTIONAL-STATES IN INTACT-CELLS
Autore/i:	Rossini, Gian Paolo; Masci, G.
Autore/i UNIMORE:	ROSSINI, Gian Paolo MASCI, G. mostra contributor esterni nascondi contributor esterni
Digital Object Identifier (DOI):	http://dx.doi.org/10.1016/0022-4731(89)90109-X
Rivista:	JOURNAL OF STEROID BIOCHEMISTRY
Volume:	34
Pagina iniziale:	363
Pagina finale:	367
Codice identificativo ISI:	WOS:A1989CN52000051
Codice identificativo Scopus:	2-s2.0-0024835135
Codice identificativo Pubmed:	2516583
Citazione:	CHEMICAL CROSSLINKING - A USEFUL TOOL FOR EVALUATIONS OF STEROID-RECEPTOR STRUCTURES AND THEIR FUNCTIONAL-STATES IN INTACT-CELLS / Rossini, Gian Paolo; Masci, G.. - In: JOURNAL OF STEROID BIOCHEMISTRY. - ISSN 0022-4731. - STAMPA. - 34(1989), pp. 363-367. [10.1016/0022-4731(89)90109-X]
Tipologia	Articolo su rivista

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