We studied, through semiquantitative PCR and in-situ hybridization, the activities of the newly identified metallothionein and phytochelatin synthase genes from the solitary ascidian Ciona intestinalis (Ci-MT and Ci-PCS, respectively) in response to 10 μM CdCl2. Metallothioneins (MTs) and phytochelatins (PCs) are involved in detoxification systems of many organisms. An appreciable number of data are available for metazoan, especially for vertebrate, but very few data are available for urochordates. As the latter occupy the peculiar phylogenetic position of invertebrate chordates, the research on MTs and PCs in tunicates assumes a particular significance. Cd strongly induced Ci-MT, with a maximum at 4 days. Ci-PCS showed maximum expression at the same time. This result is probably related to a cell proliferation event, rather than an effective Ci-PCS gene activation. The hypothesis is supported by the strong induction of Proliferating Cell Nuclear Antigen (PCNA) transcript after 4 days of treatment and the colocalization in the hemocytes of the different riboprobes used. In addition, in literature is reported an expression profile similar to that of CiMT for C. intestinalis mannose binding lectins (CiMBL). Collectively, our data and data from the literature support the conclusion that hemocyte proliferation occurs in tunicate immune responses.

Hemocyte proliferation influences metallothionein and phytochelatin levels in Ciona intestinalis / Franchi, N.; Piccinni, E.; Ballarin, L.. - In: INVERTEBRATE SURVIVAL JOURNAL. - ISSN 1824-307X. - 8:(2011), pp. 44-44. ((Intervento presentato al convegno XII Convegno della Società Italiana di Immunologia Comparata e dello Sviluppo (SIICS) tenutosi a Monteortone (PD) nel 16-18 febbraio 2011.

Hemocyte proliferation influences metallothionein and phytochelatin levels in Ciona intestinalis.

Franchi N.;
2011

Abstract

We studied, through semiquantitative PCR and in-situ hybridization, the activities of the newly identified metallothionein and phytochelatin synthase genes from the solitary ascidian Ciona intestinalis (Ci-MT and Ci-PCS, respectively) in response to 10 μM CdCl2. Metallothioneins (MTs) and phytochelatins (PCs) are involved in detoxification systems of many organisms. An appreciable number of data are available for metazoan, especially for vertebrate, but very few data are available for urochordates. As the latter occupy the peculiar phylogenetic position of invertebrate chordates, the research on MTs and PCs in tunicates assumes a particular significance. Cd strongly induced Ci-MT, with a maximum at 4 days. Ci-PCS showed maximum expression at the same time. This result is probably related to a cell proliferation event, rather than an effective Ci-PCS gene activation. The hypothesis is supported by the strong induction of Proliferating Cell Nuclear Antigen (PCNA) transcript after 4 days of treatment and the colocalization in the hemocytes of the different riboprobes used. In addition, in literature is reported an expression profile similar to that of CiMT for C. intestinalis mannose binding lectins (CiMBL). Collectively, our data and data from the literature support the conclusion that hemocyte proliferation occurs in tunicate immune responses.
XII Convegno della Società Italiana di Immunologia Comparata e dello Sviluppo (SIICS)
Monteortone (PD)
16-18 febbraio 2011
8
44
44
Franchi, N.; Piccinni, E.; Ballarin, L.
Hemocyte proliferation influences metallothionein and phytochelatin levels in Ciona intestinalis / Franchi, N.; Piccinni, E.; Ballarin, L.. - In: INVERTEBRATE SURVIVAL JOURNAL. - ISSN 1824-307X. - 8:(2011), pp. 44-44. ((Intervento presentato al convegno XII Convegno della Società Italiana di Immunologia Comparata e dello Sviluppo (SIICS) tenutosi a Monteortone (PD) nel 16-18 febbraio 2011.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1252966
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