The present paper describes the immune role played by a recently identified (Gasparini et al. 2008) member of the rhamnose-binding lectin (RBL) family from the colonial ascidian Botryllus schlosseri. B. schlosseri RBL (BsRBL) can activate phagocytes through: (i) induction of their directional movement towards the source of the molecule; (ii) modification of cytoskeleton, required for shape changes; (iii) stimulation of the respiratory burst, and consequent production of reactive oxygen species (ROS) with microbicidal activity, including superoxide anions and peroxides; and (iv) increase in the ability to phagocytose foreign particles. RBL also induces the synthesis and release, by cytotoxic morula cells (MCs), of cytokines recognised by anti-IL1_ and anti-TNF_ antibodies. At high concentrations, BsRBL induces degranulation of MCs and the consequent release of the cytotoxic enzyme phenoloxidase into the medium. Results are consistent with the existence of cross-talk between B. schlosseri immunocytes (phagocytes and MCs). In addition, a three-dimensional model for BsRBL is presented.

Immune roles of a rhamnose-binding lectin in the colonial ascidian Botryllus schlosseri / Franchi, Nicola; Schiavon, Filippo; Carletto, M; Gasparini, Fabio; Bertoloni, G; Tosatto, Silvio; Ballarin, Loriano. - In: IMMUNOBIOLOGY. - ISSN 0171-2985. - 216:(2011), pp. 725-736. [10.1016/j.imbio.2010.10.011]

Immune roles of a rhamnose-binding lectin in the colonial ascidian Botryllus schlosseri

FRANCHI, NICOLA;
2011

Abstract

The present paper describes the immune role played by a recently identified (Gasparini et al. 2008) member of the rhamnose-binding lectin (RBL) family from the colonial ascidian Botryllus schlosseri. B. schlosseri RBL (BsRBL) can activate phagocytes through: (i) induction of their directional movement towards the source of the molecule; (ii) modification of cytoskeleton, required for shape changes; (iii) stimulation of the respiratory burst, and consequent production of reactive oxygen species (ROS) with microbicidal activity, including superoxide anions and peroxides; and (iv) increase in the ability to phagocytose foreign particles. RBL also induces the synthesis and release, by cytotoxic morula cells (MCs), of cytokines recognised by anti-IL1_ and anti-TNF_ antibodies. At high concentrations, BsRBL induces degranulation of MCs and the consequent release of the cytotoxic enzyme phenoloxidase into the medium. Results are consistent with the existence of cross-talk between B. schlosseri immunocytes (phagocytes and MCs). In addition, a three-dimensional model for BsRBL is presented.
2011
216
725
736
Immune roles of a rhamnose-binding lectin in the colonial ascidian Botryllus schlosseri / Franchi, Nicola; Schiavon, Filippo; Carletto, M; Gasparini, Fabio; Bertoloni, G; Tosatto, Silvio; Ballarin, Loriano. - In: IMMUNOBIOLOGY. - ISSN 0171-2985. - 216:(2011), pp. 725-736. [10.1016/j.imbio.2010.10.011]
Franchi, Nicola; Schiavon, Filippo; Carletto, M; Gasparini, Fabio; Bertoloni, G; Tosatto, Silvio; Ballarin, Loriano
File in questo prodotto:
File Dimensione Formato  
2011 Immunobiology (BsRBL immune roles).pdf

Accesso riservato

Dimensione 1.43 MB
Formato Adobe PDF
1.43 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
2011 Immunobiology (BsRBL immune roles).pdf

Accesso riservato

Dimensione 1.43 MB
Formato Adobe PDF
1.43 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1252894
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 33
social impact