Increasing evidences indicate that functional, non-toxic amyloid is widely distributed among living organisms. In invertebrates and vertebrates, functional amyloid is involved in inflammatory reactions and modulation of immune responses. In the present study, we investigated the occurrence of functional amyloid in tunicates, the closest living relatives of vertebrates. Previous studies indicate that orthologous genes for the amyloid precursor protein (APP), involved in amyloid synthesis in vertebrates, are present in solitary ascidians of the genus Ciona. In addition, colonies of compound ascidians of the genus Botryllus, respond to contacting genetically incompatible colonies with an allorecognition reaction that leads to the formation of necrotic, melanic spots along the contact border. Our data provide evidence that functional amyloid is involved in immune responses of Botryllus schlosseri and indicate that, both the circulating immunocyte types, i.e., the cytotoxic morula cells and phagocytes, can produce amyloid using two different proteins: Bsp102 and BsAPP. Bsp102 forms the amyloid functioning as scaffold to store MC granular content and, once released upon MC degranulation, the support where phenoloxidase and melanin are deposited, thus limiting the diffusion of cytotoxicity. BsAPP is released by phagocytes and contribute to the formation of extracellular nets that entrap microbes and prevent their diffusion within the organism. To the best of our knowledge, this is the first report of functional amyloidogenesis in protochordate immunity.

Amyloid and immune responses in the colonial ascidian Botryllus schlosseri / Ballarin, L.; Franchi, N.; Peronato, A.; Grimaldi, A.; Girardello, R.; de Eguileor, M. - In: INVERTEBRATE SURVIVAL JOURNAL. - ISSN 1824-307X. - 15:(2018), pp. 124-124.

Amyloid and immune responses in the colonial ascidian Botryllus schlosseri

Franchi N.;
2018

Abstract

Increasing evidences indicate that functional, non-toxic amyloid is widely distributed among living organisms. In invertebrates and vertebrates, functional amyloid is involved in inflammatory reactions and modulation of immune responses. In the present study, we investigated the occurrence of functional amyloid in tunicates, the closest living relatives of vertebrates. Previous studies indicate that orthologous genes for the amyloid precursor protein (APP), involved in amyloid synthesis in vertebrates, are present in solitary ascidians of the genus Ciona. In addition, colonies of compound ascidians of the genus Botryllus, respond to contacting genetically incompatible colonies with an allorecognition reaction that leads to the formation of necrotic, melanic spots along the contact border. Our data provide evidence that functional amyloid is involved in immune responses of Botryllus schlosseri and indicate that, both the circulating immunocyte types, i.e., the cytotoxic morula cells and phagocytes, can produce amyloid using two different proteins: Bsp102 and BsAPP. Bsp102 forms the amyloid functioning as scaffold to store MC granular content and, once released upon MC degranulation, the support where phenoloxidase and melanin are deposited, thus limiting the diffusion of cytotoxicity. BsAPP is released by phagocytes and contribute to the formation of extracellular nets that entrap microbes and prevent their diffusion within the organism. To the best of our knowledge, this is the first report of functional amyloidogenesis in protochordate immunity.
15
124
124
Ballarin, L.; Franchi, N.; Peronato, A.; Grimaldi, A.; Girardello, R.; de Eguileor, M
Amyloid and immune responses in the colonial ascidian Botryllus schlosseri / Ballarin, L.; Franchi, N.; Peronato, A.; Grimaldi, A.; Girardello, R.; de Eguileor, M. - In: INVERTEBRATE SURVIVAL JOURNAL. - ISSN 1824-307X. - 15:(2018), pp. 124-124.
File in questo prodotto:
File Dimensione Formato  
ISJ SIICS 2018_2.pdf

non disponibili

Dimensione 1.04 MB
Formato Adobe PDF
1.04 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1252889
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact