Sodium arsenite proved effective in preventing the induction of ornithine decarboxylase (ODC) activity elicited by dilution of Friend erythroleukemia cells in fresh medium. A 50 per cent inhibition was produced at approximately 1 μM arsenite and complete inhibition was obtained at concentrations above 10 μM. However, addition of arsenite 5 h after cell dilution, i.e. when ODC was already induced, appeared to stabilize the enzyme. The half‐life of ODC activity, measured after cycloheximide treatment, increased almost six‐fold after addition of sodium arsenite. Agents known to provoke oxidative alteration of the thiol‐redox status in cells, also caused a similar effect on the induction and stability of ODC. Copyright © 1989 John Wiley & Sons Ltd.
Effect of sodium arsentie on the induction and turnover of ornithine decarboxylase activity in erythroleukemia cells / Flamigni, F.; Marmiroli, S.; Caldarera, C. M.; Guarnieri, C.. - In: CELL BIOCHEMISTRY AND FUNCTION. - ISSN 0263-6484. - 7:3(1989), pp. 213-217. [10.1002/cbf.290070310]
Effect of sodium arsentie on the induction and turnover of ornithine decarboxylase activity in erythroleukemia cells
Marmiroli S.;
1989
Abstract
Sodium arsenite proved effective in preventing the induction of ornithine decarboxylase (ODC) activity elicited by dilution of Friend erythroleukemia cells in fresh medium. A 50 per cent inhibition was produced at approximately 1 μM arsenite and complete inhibition was obtained at concentrations above 10 μM. However, addition of arsenite 5 h after cell dilution, i.e. when ODC was already induced, appeared to stabilize the enzyme. The half‐life of ODC activity, measured after cycloheximide treatment, increased almost six‐fold after addition of sodium arsenite. Agents known to provoke oxidative alteration of the thiol‐redox status in cells, also caused a similar effect on the induction and stability of ODC. Copyright © 1989 John Wiley & Sons Ltd.
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