We have investigated the structure of the human HOX-2 locus, which encompasses a 90-kb region on chromosome 17q21. Five new human HOX-2 homeoboxes, termed HOX-2.5, 2.4, 2.6, 2.7 and 2.8, Have been identified, and their nucleotide sequences are reported. They have the same 5′–3′transcriptional korientation and are clustered with three previously described HOX-2 homeoboxes (5′–2.5–2.4–2.3–2.2-2.1–2.6–2.7–2.8-3′). We have also investigated the region-specific expression of HOX-2 genes in human embryonic-fetal life by Northern-blot analysis. All genes are expressed in whole embrvos and fetuses at 5–9 weeks from conception. Their major site of expression lies within the central nervous system (CNS), althought they are transcribed at a lower level in body structures other than the CNS. Their relatively abundant experession in CNS has been analyzed along the anterior-posterior axis by dissecting the brain, the Medulla oblongata and the spinal cord proper. HOX-2.5, 2.4 and 2.3 transcripts are markedly more abundant in spinal cord than in medulla, whereas 2.2, 2.2, 2.6 and 2.7 mRNAs are progressively more abundant in the medull. Additionall, expression in brain was detected, although at lower level, for HOX-2.1, 2.7, 2.8. Thus, the relative position of HOX-2 homeobox genes along the chromosome in the 5′–3′direction appears to correlate with CNS longitudinal axis in the caudal-cephalic direction. © 1989, International Society of Differentiation. All rights reserved.
Differential expression of human HOX-2 genes along the anterior-posterior axis in embryonic central nervous system / Giampaolo, A.; Acampora, D.; Zappavigna, V.; Pannese, M.; D'Esposito, M.; Care, A.; Feiella, A.; Stornaiuolo, A.; Russo, G.; Simeone, A.; Boncinelli, E.; Peschle, C.. - In: DIFFERENTIATION. - ISSN 0301-4681. - 40:3(1989), pp. 191-197. [10.1111/j.1432-0436.1989.tb00598.x]
Differential expression of human HOX-2 genes along the anterior-posterior axis in embryonic central nervous system
Zappavigna V.;
1989
Abstract
We have investigated the structure of the human HOX-2 locus, which encompasses a 90-kb region on chromosome 17q21. Five new human HOX-2 homeoboxes, termed HOX-2.5, 2.4, 2.6, 2.7 and 2.8, Have been identified, and their nucleotide sequences are reported. They have the same 5′–3′transcriptional korientation and are clustered with three previously described HOX-2 homeoboxes (5′–2.5–2.4–2.3–2.2-2.1–2.6–2.7–2.8-3′). We have also investigated the region-specific expression of HOX-2 genes in human embryonic-fetal life by Northern-blot analysis. All genes are expressed in whole embrvos and fetuses at 5–9 weeks from conception. Their major site of expression lies within the central nervous system (CNS), althought they are transcribed at a lower level in body structures other than the CNS. Their relatively abundant experession in CNS has been analyzed along the anterior-posterior axis by dissecting the brain, the Medulla oblongata and the spinal cord proper. HOX-2.5, 2.4 and 2.3 transcripts are markedly more abundant in spinal cord than in medulla, whereas 2.2, 2.2, 2.6 and 2.7 mRNAs are progressively more abundant in the medull. Additionall, expression in brain was detected, although at lower level, for HOX-2.1, 2.7, 2.8. Thus, the relative position of HOX-2 homeobox genes along the chromosome in the 5′–3′direction appears to correlate with CNS longitudinal axis in the caudal-cephalic direction. © 1989, International Society of Differentiation. All rights reserved.
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