A microscopic analysis of tissue is the gold standard for cancer detection. Hematoxylin-eosin (HE) for the reporting of prostate biopsy (PB) is conventionally based on fixation, processing, acquisition of glass slides, and analysis with an analog microscope by a local pathologist. Digitalization and real-time remote access to images could enhance the reporting process, and form the basis of artificial intelligence and machine learning. Fluorescence confocal microscopy (FCM), a novel optical technology, enables immediate digital image acquisition in an almost HE-like resolution without requiring conventional processing.
Digital Biopsy with Fluorescence Confocal Microscope for Effective Real-time Diagnosis of Prostate Cancer: A Prospective, Comparative Study / Rocco, B., Sighinolfi, M.C., Sandri, M., Spandri, V., Cimadamore, A., Volavsek, M., Mazzucchelli, R., Lopez-Beltran, A., Eissa, A., Bertoni, L., Azzoni, P., Reggiani Bonetti, L., Maiorana, A., Puliatti, S., Micali, S., Paterlini, M., Iseppi, A., Rocco, F., Pellacani, G., Chester, J., et al.. - In: EUROPEAN UROLOGY ONCOLOGY. - ISSN 2588-9311. - 4:5(2021), pp. 784-791. [10.1016/j.euo.2020.08.009]
Digital Biopsy with Fluorescence Confocal Microscope for Effective Real-time Diagnosis of Prostate Cancer: A Prospective, Comparative Study
Rocco, Bernardo;Sighinolfi, Maria Chiara;Sandri, Marco;Bertoni, Laura;Azzoni, Paola;Reggiani Bonetti, Luca;Maiorana, Antonino;Puliatti, Stefano;Micali, Salvatore;Iseppi, Andrea;Pellacani, Giovanni;Chester, Johanna;Bianchi, Giampaolo;
2021
Abstract
A microscopic analysis of tissue is the gold standard for cancer detection. Hematoxylin-eosin (HE) for the reporting of prostate biopsy (PB) is conventionally based on fixation, processing, acquisition of glass slides, and analysis with an analog microscope by a local pathologist. Digitalization and real-time remote access to images could enhance the reporting process, and form the basis of artificial intelligence and machine learning. Fluorescence confocal microscopy (FCM), a novel optical technology, enables immediate digital image acquisition in an almost HE-like resolution without requiring conventional processing.
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