In the insect Lymantria dispar cell line IPLB-LdFB the presence of a Bcl-2-like molecule has been demonstrated. The Western blot analysis performed on the cells incubated with 2-deoxy-D-ribose (dRib), an apoptotic inducer, revealed that, in comparison with the control, the Bcl-2 expression was unaffected. Furthermore, incubation of the insect cells with an anti-Bcl-2 polyclonal antibody inhibited the apoptotic effect induced by dRib, and provoked mitochondrial membrane depolarization without any apoptotic phenomena. Similar behaviour was observed using the K+ ionophore valinomycin. From these findings, we hypothesize that the L. dispar Bcl-2-like protein is essential for maintenance of the mitochondrial membrane potential, but not, as usually thought, for the regulation of programmed cell death. (C) 2002 Elsevier Science Ltd. All rights reserved.

In the insect Lymantria dispar cell line IPLB-LdFB the presence of a Bcl-2-1ike molecule has been demonstrated. The Western blot analysis performed on the cells incubated with 2-deoxy-D-ribose (dRib), an apoptotic inducer, revealed that, in comparison with the control, the Bcl-2 expression was unaffected. Furthermore, incubation of the insect cells with an anti-Bcl-2 polyclonal antibody inhibited the apoptotic effect induced by dRib, and provoked mitochondrial membrane depolarization without any apoptotic phenomena. Similar behaviour was observed using the K+ ionophore valinomycin. From these findings, we hypothesize that the L. dispar Bcl-2-1ike protein is essential for maintenance of the mitochondrial membrane potential, but not, as usually thought, for the regulation of programmed cell death. © 2002 Elsevier Science Ltd. All rights reserved.

Bcl-2 does not control programmed cell death in the IPLB-LdFB cell line from the insect Lymantria dispar / Ottaviani, E.; Malagoli, D.. - In: CELL BIOLOGY INTERNATIONAL. - ISSN 1065-6995. - 26:6(2002), pp. 563-566. [10.1006/cbir.2002.0885]

Bcl-2 does not control programmed cell death in the IPLB-LdFB cell line from the insect Lymantria dispar

Ottaviani E.;Malagoli D.
2002

Abstract

In the insect Lymantria dispar cell line IPLB-LdFB the presence of a Bcl-2-1ike molecule has been demonstrated. The Western blot analysis performed on the cells incubated with 2-deoxy-D-ribose (dRib), an apoptotic inducer, revealed that, in comparison with the control, the Bcl-2 expression was unaffected. Furthermore, incubation of the insect cells with an anti-Bcl-2 polyclonal antibody inhibited the apoptotic effect induced by dRib, and provoked mitochondrial membrane depolarization without any apoptotic phenomena. Similar behaviour was observed using the K+ ionophore valinomycin. From these findings, we hypothesize that the L. dispar Bcl-2-1ike protein is essential for maintenance of the mitochondrial membrane potential, but not, as usually thought, for the regulation of programmed cell death. © 2002 Elsevier Science Ltd. All rights reserved.
2002
26
6
563
566
Bcl-2 does not control programmed cell death in the IPLB-LdFB cell line from the insect Lymantria dispar / Ottaviani, E.; Malagoli, D.. - In: CELL BIOLOGY INTERNATIONAL. - ISSN 1065-6995. - 26:6(2002), pp. 563-566. [10.1006/cbir.2002.0885]
Ottaviani, E.; Malagoli, D.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1247991
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