The active transport of serotonin (5-HT) was studied in two cultured cell lines; FET (a finite fibroblast-like cell line derived from the osteomuscular structures of C3H embryo mice) and EUE (a continuous cell line derived from a human embryonal epithelium). These transport mechanisms are characterized by a high affinity carrier mediated system (km approx 1.3 uM and 1.0 uM respectively; Vmax approx 8 pmol/20 min × 106 cells and 2 pmol/20 min × 106 cells respectively) and temperature dependence. Fluoxetine (Lilly 110140) and Chlorimipramine preferentially block FET but not EUE serotonin uptake. Our results suggest that a serotonin-like transport system is present in unspecialized cultured cells, but it differs kinetically from the neuronal serotonin transport mechanism. © 1982 The Italian Pharmacological Society.
Studies on a neuronal-like transport system for serotonin in two cell lines / Vaghi, M.; Curatolo, L.; Algeri, S.; Brunello, N.; Mennini, T.; Morasca, L.; Salmona, A. L.; Mella, L.; Locati, M.; Dolfini, E.. - In: PHARMACOLOGICAL RESEARCH COMMUNICATIONS. - ISSN 0031-6989. - 14:9(1982), pp. 851-859. [10.1016/S0031-6989(82)80009-X]