There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(i) or platinum(ii) in the current study, enables techniques such as nanoSIMS. While the rhenium(i) complex was not sufficiently stable to be used as a probe, the platinum(ii) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(ii) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria.

Structure illumination microscopy imaging of lipid vesicles in live bacteria with naphthalimide-appended organometallic complexes / Maria Ranieri, Anna; Vezzelli, Matteo; Leslie, Kathryn G; Huang, Song; Stagni, Stefano; Jacquemin, Denis; Jiang, Haibo; Hubbard, Alysia; Rigamonti, Luca; Watkin, Elizabeth L J; Ogden, Mark I; New, Elizabeth J; Massi, Massimiliano. - In: ANALYST. - ISSN 0003-2654. - 146:(2021), pp. 3818-3822. [10.1039/d1an00363a]

Structure illumination microscopy imaging of lipid vesicles in live bacteria with naphthalimide-appended organometallic complexes

Vezzelli, Matteo;Rigamonti, Luca;
2021

Abstract

There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(i) or platinum(ii) in the current study, enables techniques such as nanoSIMS. While the rhenium(i) complex was not sufficiently stable to be used as a probe, the platinum(ii) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(ii) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria.
17-mag-2021
146
3818
3822
Structure illumination microscopy imaging of lipid vesicles in live bacteria with naphthalimide-appended organometallic complexes / Maria Ranieri, Anna; Vezzelli, Matteo; Leslie, Kathryn G; Huang, Song; Stagni, Stefano; Jacquemin, Denis; Jiang, Haibo; Hubbard, Alysia; Rigamonti, Luca; Watkin, Elizabeth L J; Ogden, Mark I; New, Elizabeth J; Massi, Massimiliano. - In: ANALYST. - ISSN 0003-2654. - 146:(2021), pp. 3818-3822. [10.1039/d1an00363a]
Maria Ranieri, Anna; Vezzelli, Matteo; Leslie, Kathryn G; Huang, Song; Stagni, Stefano; Jacquemin, Denis; Jiang, Haibo; Hubbard, Alysia; Rigamonti, Luca; Watkin, Elizabeth L J; Ogden, Mark I; New, Elizabeth J; Massi, Massimiliano
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1247549
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