Intestinal cells from chicken embryos were grown in chemically defined, serum-free medium. The majority of cultured cells exhibits an epithelial-like morphology. As demonstrated by indirect immunofluorescence, the epithelial cells, and not the contaminating fibroblasts, express Calbindin-D28K only after 1,25-dihydroxyvitamin D3, the hormonally active form of vitamin D, is added to the culture medium. The highly sensitive reverse transcriptase-polymerase chain reaction shows that both Calbindin-D28K mRNA and the corresponding primary unprocessed transcripts (pre-mRNA) are dramatically increased in cultured intestinal cells treated with 1,25-dihydroxyvitamin D3, thus indicating that Calbindin-D28K is induced by the increased rate of transcription of the corresponding gene. © 1992.

Induction of Calbindin-D28K by 1,25-dihydroxyvitamin D3 in cultured chicken intestinal cells / Ferrari, Stefano; Molinari, Susanna; Battini, Renata; Cossu, G; Lamonfava, S.. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 200:2(1992), pp. 528-531. [10.1016/0014-4827(92)90205-M]

Induction of Calbindin-D28K by 1,25-dihydroxyvitamin D3 in cultured chicken intestinal cells

FERRARI, Stefano;MOLINARI, Susanna;BATTINI, Renata;
1992

Abstract

Intestinal cells from chicken embryos were grown in chemically defined, serum-free medium. The majority of cultured cells exhibits an epithelial-like morphology. As demonstrated by indirect immunofluorescence, the epithelial cells, and not the contaminating fibroblasts, express Calbindin-D28K only after 1,25-dihydroxyvitamin D3, the hormonally active form of vitamin D, is added to the culture medium. The highly sensitive reverse transcriptase-polymerase chain reaction shows that both Calbindin-D28K mRNA and the corresponding primary unprocessed transcripts (pre-mRNA) are dramatically increased in cultured intestinal cells treated with 1,25-dihydroxyvitamin D3, thus indicating that Calbindin-D28K is induced by the increased rate of transcription of the corresponding gene. © 1992.
200
2
528
531
Induction of Calbindin-D28K by 1,25-dihydroxyvitamin D3 in cultured chicken intestinal cells / Ferrari, Stefano; Molinari, Susanna; Battini, Renata; Cossu, G; Lamonfava, S.. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 200:2(1992), pp. 528-531. [10.1016/0014-4827(92)90205-M]
Ferrari, Stefano; Molinari, Susanna; Battini, Renata; Cossu, G; Lamonfava, S.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1247498
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