The efficacy of immunotherapy can be undermined by the development of an immune response against a drug/antibody mediated by anti-drug antibodies (ADAs) in treated patients. We present the first label-free EGOFET immunosensor that integrates a biological drug, Nivolumab (Opdivo©), as a specific recognition moiety to quantitatively and selectively detect ADAs against the drug. The limit of detection is 100 fM. This demonstration is a prelude to the detection of ADAs in a clinical setting in the treatment of different pathologies, and it also enables rapid screening of biological drugs for immunogenicity.

Anti-drug antibody detection with label-free electrolyte-gated organic field-effect transistors / Sensi, Matteo; Berto, Marcello; Gentile, Sara; Pinti, Marcello; Conti, Andrea; Pellacani, Giovanni; Salvarani, Carlo; Cossarizza, Andrea; Bortolotti, Carlo Augusto; Biscarini, Fabio. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - 57:3(2021), pp. 367-370. [10.1039/d0cc03399e]

Anti-drug antibody detection with label-free electrolyte-gated organic field-effect transistors

Sensi Matteo;Berto Marcello;Gentile Sara;Pinti Marcello;Salvarani Carlo;Cossarizza AndreA;Bortolotti Carlo Augusto;Biscarini Fabio
2021

Abstract

The efficacy of immunotherapy can be undermined by the development of an immune response against a drug/antibody mediated by anti-drug antibodies (ADAs) in treated patients. We present the first label-free EGOFET immunosensor that integrates a biological drug, Nivolumab (Opdivo©), as a specific recognition moiety to quantitatively and selectively detect ADAs against the drug. The limit of detection is 100 fM. This demonstration is a prelude to the detection of ADAs in a clinical setting in the treatment of different pathologies, and it also enables rapid screening of biological drugs for immunogenicity.
16-dic-2020
57
3
367
370
Anti-drug antibody detection with label-free electrolyte-gated organic field-effect transistors / Sensi, Matteo; Berto, Marcello; Gentile, Sara; Pinti, Marcello; Conti, Andrea; Pellacani, Giovanni; Salvarani, Carlo; Cossarizza, Andrea; Bortolotti, Carlo Augusto; Biscarini, Fabio. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - 57:3(2021), pp. 367-370. [10.1039/d0cc03399e]
Sensi, Matteo; Berto, Marcello; Gentile, Sara; Pinti, Marcello; Conti, Andrea; Pellacani, Giovanni; Salvarani, Carlo; Cossarizza, Andrea; Bortolotti, Carlo Augusto; Biscarini, Fabio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1247491
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