The present work describes the construction of a cDNA library in pBR322 plasmid from an mRNA population enriched for the intestinal calcium-binding protein (CaBP) mRNA of the chick. We report the isolation of one recombinant clone containing a vitamin D-regulated sequence, which is complementary to part of the CaBP mRNA. Northern blot hybridization experiments allowed us to identify a 1900 nucleotide RNA species as the CaBP mRNA. © 1984.
Isolation of a cDNA clone containing a sequence complementary to the intestinal calcium-binding protein of the chick / Ferrari, S.; Battini, R.; Leone, A.; Ferrari, S.; Torelli, G.; Barbiroli, B.. - In: GENE. - ISSN 0378-1119. - 30:1-3(1984), pp. 233-236. [10.1016/0378-1119(84)90125-2]
Isolation of a cDNA clone containing a sequence complementary to the intestinal calcium-binding protein of the chick
Ferrari S.;Battini R.;Ferrari S.;Torelli G.;
1984
Abstract
The present work describes the construction of a cDNA library in pBR322 plasmid from an mRNA population enriched for the intestinal calcium-binding protein (CaBP) mRNA of the chick. We report the isolation of one recombinant clone containing a vitamin D-regulated sequence, which is complementary to part of the CaBP mRNA. Northern blot hybridization experiments allowed us to identify a 1900 nucleotide RNA species as the CaBP mRNA. © 1984.Pubblicazioni consigliate
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