The dimorphic transition of Candida albicans from the yeast (Y-Candida) to the hyphal (H-Candida) form is a complex event whose relevance in fungal pathogenicity is still poorly understood. Using a cloned macrophage (Mφ) cell line (ANA-1), we have previously shown that a Mφ can discriminate between the two fungal forms, eliciting different secretory responses. In the present study, we investigated the susceptibility of Y-Candida and H-Candida to Mφ proteolytic activity. In particular, sodium dodecyl sulfate- polyacrylamide gel electrophoresis and Western blot (immunoblot) techniques were employed to analyze the patterns of lyticase proteinaceous extracts from cell walls of Y-Candida and H-Candida which had been unexposed or exposed to ANA-1 Mφs for 3 h. Silver staining allowed detection of a complex protein pattern in both forms of C. albicans, qualitatively and quantitatively differing from each other, mainly at molecular masses below 106 kDa. Western blot staining with anti-C. albicans mannan antibodies and convalescent-phase sera of mice previously infected systemically or intracerebrally with C. albicans showed that, after contact with Mφs, Y-Candida but not H-Candida proteinaceous cell wall components are profoundly modified, with substantial reduction and/or disappearance of many bands. Our experimental approach provides initial insights into the differential susceptibility of Y-Candida and H-Candida to the proteolytic activity of Mφs.

Differential susceptibility of yeast and hyphal forms of Candida albicans to proteolytic activity of macrophages / Blasi, E.; Pitzurra, L.; Chimienti, A. R.; Mazzolla, R.; Puliti, M.; Barluzzi, R.; Bistoni, F.. - In: INFECTION AND IMMUNITY. - ISSN 0019-9567. - 63:4(1995), pp. 1253-1257. [10.1128/iai.63.4.1253-1257.1995]

Differential susceptibility of yeast and hyphal forms of Candida albicans to proteolytic activity of macrophages

Blasi E.;
1995

Abstract

The dimorphic transition of Candida albicans from the yeast (Y-Candida) to the hyphal (H-Candida) form is a complex event whose relevance in fungal pathogenicity is still poorly understood. Using a cloned macrophage (Mφ) cell line (ANA-1), we have previously shown that a Mφ can discriminate between the two fungal forms, eliciting different secretory responses. In the present study, we investigated the susceptibility of Y-Candida and H-Candida to Mφ proteolytic activity. In particular, sodium dodecyl sulfate- polyacrylamide gel electrophoresis and Western blot (immunoblot) techniques were employed to analyze the patterns of lyticase proteinaceous extracts from cell walls of Y-Candida and H-Candida which had been unexposed or exposed to ANA-1 Mφs for 3 h. Silver staining allowed detection of a complex protein pattern in both forms of C. albicans, qualitatively and quantitatively differing from each other, mainly at molecular masses below 106 kDa. Western blot staining with anti-C. albicans mannan antibodies and convalescent-phase sera of mice previously infected systemically or intracerebrally with C. albicans showed that, after contact with Mφs, Y-Candida but not H-Candida proteinaceous cell wall components are profoundly modified, with substantial reduction and/or disappearance of many bands. Our experimental approach provides initial insights into the differential susceptibility of Y-Candida and H-Candida to the proteolytic activity of Mφs.
1995
63
4
1253
1257
Differential susceptibility of yeast and hyphal forms of Candida albicans to proteolytic activity of macrophages / Blasi, E.; Pitzurra, L.; Chimienti, A. R.; Mazzolla, R.; Puliti, M.; Barluzzi, R.; Bistoni, F.. - In: INFECTION AND IMMUNITY. - ISSN 0019-9567. - 63:4(1995), pp. 1253-1257. [10.1128/iai.63.4.1253-1257.1995]
Blasi, E.; Pitzurra, L.; Chimienti, A. R.; Mazzolla, R.; Puliti, M.; Barluzzi, R.; Bistoni, F.
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