The chromium release technique for quantifying intracellular killing of radiolabelled Candida albicans particles was exploited in a microassay in which murine and human phagocytes acted as effectors under peculiarly simple conditions. At appropriate effector: target ratios and with a 4 h incubation, up to 50% specific chromium release could be detected in the supernatant with no need for opsonization or lysis of phagocytes. This simple microassay permits easy-to-perform, simultaneous testing of a variety of different phagocytes even if only available in limited amounts, and provides an objective measurement of intracellular killing of Candida albicans. © 1982.

A radiolabel release microassay for phagocytic killing of Candida albicans / Bistoni, F.; Baccarini, M.; Blasi, E.; Puccetti, P.; Marconi, P.. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 52:3(1982), pp. 369-377. [10.1016/0022-1759(82)90008-4]

A radiolabel release microassay for phagocytic killing of Candida albicans

Blasi E.;
1982

Abstract

The chromium release technique for quantifying intracellular killing of radiolabelled Candida albicans particles was exploited in a microassay in which murine and human phagocytes acted as effectors under peculiarly simple conditions. At appropriate effector: target ratios and with a 4 h incubation, up to 50% specific chromium release could be detected in the supernatant with no need for opsonization or lysis of phagocytes. This simple microassay permits easy-to-perform, simultaneous testing of a variety of different phagocytes even if only available in limited amounts, and provides an objective measurement of intracellular killing of Candida albicans. © 1982.
1982
52
3
369
377
A radiolabel release microassay for phagocytic killing of Candida albicans / Bistoni, F.; Baccarini, M.; Blasi, E.; Puccetti, P.; Marconi, P.. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 52:3(1982), pp. 369-377. [10.1016/0022-1759(82)90008-4]
Bistoni, F.; Baccarini, M.; Blasi, E.; Puccetti, P.; Marconi, P.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1247206
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