Secretion of the lysosomal enzyme β-N-acetylhexosaminidase is inhibited by calcium ionophore A-23187 in the GG2EE macrophage cell line. Such inhibition is time and dose dependent. Calcium ionophore A-23187 treatment causes a change in the pattern of hexosaminidase isoenzymes detectable in the cell extract, as assessed by DEAE-cellulose chromatography. In particular, control cells show two hexosaminidase isoenzymes corresponding to hexosaminidase A and B, whereas cells treated with calcium ionophore A-23187 express a third isoenzyme form with properties similar to hexosaminidase S.
Calcium ionophore A-23187 inhibits the secretion of β-hexosaminidase from the GG2EE mouse macrophage cell line / Beccari, T.; Datti, A.; Orlacchio, A.; Farinelli, S.; Blasi, E.. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - 27:5(1992), pp. 783-791.
Calcium ionophore A-23187 inhibits the secretion of β-hexosaminidase from the GG2EE mouse macrophage cell line
Blasi E.
1992
Abstract
Secretion of the lysosomal enzyme β-N-acetylhexosaminidase is inhibited by calcium ionophore A-23187 in the GG2EE macrophage cell line. Such inhibition is time and dose dependent. Calcium ionophore A-23187 treatment causes a change in the pattern of hexosaminidase isoenzymes detectable in the cell extract, as assessed by DEAE-cellulose chromatography. In particular, control cells show two hexosaminidase isoenzymes corresponding to hexosaminidase A and B, whereas cells treated with calcium ionophore A-23187 express a third isoenzyme form with properties similar to hexosaminidase S.Pubblicazioni consigliate
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