Microdensitometric and morphometric techniques have been developed to quantitatively characterize cell groups and terminal populations of transmitter-identified neuronal systems. Various microdensitometric methods implemented on the image analyzer or on the scanning microdensitometer were introduced and compared. On this basis a technique to assess the half-life of dopamine stores determined by quantitative immunocytochemistry has been developed. The problem of relative and absolute quantification of microdensitometric analysis of immunocytochemical preparations is discussed here. A method has been developed for the study, both in 2- and 3-dimensions, of the overall features of the profile distribution in a defined neuroanatomical area. An approach to determine the degree of uniformity of a certain profile distribution is also proposed. Furthermore, methods for the evaluation of the codistribution of two or more different types of profiles and to characterize the morphometric features of patches of profiles in a certain region are presented. All these quantitative morphological approaches were tested in relevant preparations of the central nervous system.

ASPECTS OF NEURAL PLASTICITY IN THE CENTRAL-NERVOUS-SYSTEM .1. COMPUTER-ASSISTED IMAGE-ANALYSIS METHODS / Zoli, Michele; Zini, Isabella; Agnati, Luigi Francesco; Guidolin, D; Ferraguti, F; Fuxe, K.. - In: NEUROCHEMISTRY INTERNATIONAL. - ISSN 0197-0186. - STAMPA. - 16:(1990), pp. 383-418.

ASPECTS OF NEURAL PLASTICITY IN THE CENTRAL-NERVOUS-SYSTEM .1. COMPUTER-ASSISTED IMAGE-ANALYSIS METHODS

ZOLI, Michele;ZINI, Isabella;AGNATI, Luigi Francesco;FERRAGUTI F;
1990

Abstract

Microdensitometric and morphometric techniques have been developed to quantitatively characterize cell groups and terminal populations of transmitter-identified neuronal systems. Various microdensitometric methods implemented on the image analyzer or on the scanning microdensitometer were introduced and compared. On this basis a technique to assess the half-life of dopamine stores determined by quantitative immunocytochemistry has been developed. The problem of relative and absolute quantification of microdensitometric analysis of immunocytochemical preparations is discussed here. A method has been developed for the study, both in 2- and 3-dimensions, of the overall features of the profile distribution in a defined neuroanatomical area. An approach to determine the degree of uniformity of a certain profile distribution is also proposed. Furthermore, methods for the evaluation of the codistribution of two or more different types of profiles and to characterize the morphometric features of patches of profiles in a certain region are presented. All these quantitative morphological approaches were tested in relevant preparations of the central nervous system.
1990
16
383
418
ASPECTS OF NEURAL PLASTICITY IN THE CENTRAL-NERVOUS-SYSTEM .1. COMPUTER-ASSISTED IMAGE-ANALYSIS METHODS / Zoli, Michele; Zini, Isabella; Agnati, Luigi Francesco; Guidolin, D; Ferraguti, F; Fuxe, K.. - In: NEUROCHEMISTRY INTERNATIONAL. - ISSN 0197-0186. - STAMPA. - 16:(1990), pp. 383-418.
Zoli, Michele; Zini, Isabella; Agnati, Luigi Francesco; Guidolin, D; Ferraguti, F; Fuxe, K.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/12418
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