Haemoglobin switching in humans provides a unique model for investigating the mechanisms underlying expression of a develop-mentally regulated gene family. Numerous studies have focused on the switch from fetal to adult (that is, γ → β) globin1,2, but little is known about the embryonic → fetal (that is, ζ → α and ε → γ) switches, as well as the transition from 'primitive' yolk sac to 'definitive' liver erythropoiesis3-7. Here we have studied the embryonic→fetal haemoglobin switches in yolk sac, liver and circulating blood erythroblasts from 25 embryos and 6 fetuses. Globin synthesis was also evaluated in purified 'primitive' and 'definitive' erythroblasts. Primitive erythroblasts synthesize essentially ζ and ε chains at 5 weeks and α- and ε-globin with a minor aliquot of ζ and γ chains at 6-7 weeks, whereas definitive erythroblasts produce α and γ + β-globin at 6 weeks but only α and γ + β chains from 8 weeks onward. In both lineages the ζ → α and the ε → γ switches are asynchronous, the former preceding the latter. Furthermore, ζ- and β-globin synthesis is restricted to primitive and definitive erythroblasts respectively. These findings are discussed in terms of a monoclonal model for haemoglobin switching in early human ontogeny. © 1985 Nature Publishing Group.

Haemoglobin switching in human embryos: Asynchrony of ζ → α and ε → γ-globin switches in primitive and definitive erythropoietic lineage / Peschle, C.; Mavilio, F.; Care, A.; Migliaccio, G.; Migliaccio, A. R.; Salvo, G.; Samoggia, P.; Petti, S.; Guerriero, R.; Marinucci, M.; Lazzaro, D.; Russo, G.; Mastroberardino, G.. - In: NATURE. - ISSN 0028-0836. - 313:5999(1985), pp. 235-238. [10.1038/313235a0]

Haemoglobin switching in human embryos: Asynchrony of ζ → α and ε → γ-globin switches in primitive and definitive erythropoietic lineage

Mavilio F.;
1985

Abstract

Haemoglobin switching in humans provides a unique model for investigating the mechanisms underlying expression of a develop-mentally regulated gene family. Numerous studies have focused on the switch from fetal to adult (that is, γ → β) globin1,2, but little is known about the embryonic → fetal (that is, ζ → α and ε → γ) switches, as well as the transition from 'primitive' yolk sac to 'definitive' liver erythropoiesis3-7. Here we have studied the embryonic→fetal haemoglobin switches in yolk sac, liver and circulating blood erythroblasts from 25 embryos and 6 fetuses. Globin synthesis was also evaluated in purified 'primitive' and 'definitive' erythroblasts. Primitive erythroblasts synthesize essentially ζ and ε chains at 5 weeks and α- and ε-globin with a minor aliquot of ζ and γ chains at 6-7 weeks, whereas definitive erythroblasts produce α and γ + β-globin at 6 weeks but only α and γ + β chains from 8 weeks onward. In both lineages the ζ → α and the ε → γ switches are asynchronous, the former preceding the latter. Furthermore, ζ- and β-globin synthesis is restricted to primitive and definitive erythroblasts respectively. These findings are discussed in terms of a monoclonal model for haemoglobin switching in early human ontogeny. © 1985 Nature Publishing Group.
1985
313
5999
235
238
Haemoglobin switching in human embryos: Asynchrony of ζ → α and ε → γ-globin switches in primitive and definitive erythropoietic lineage / Peschle, C.; Mavilio, F.; Care, A.; Migliaccio, G.; Migliaccio, A. R.; Salvo, G.; Samoggia, P.; Petti, S.; Guerriero, R.; Marinucci, M.; Lazzaro, D.; Russo, G.; Mastroberardino, G.. - In: NATURE. - ISSN 0028-0836. - 313:5999(1985), pp. 235-238. [10.1038/313235a0]
Peschle, C.; Mavilio, F.; Care, A.; Migliaccio, G.; Migliaccio, A. R.; Salvo, G.; Samoggia, P.; Petti, S.; Guerriero, R.; Marinucci, M.; Lazzaro, D.; ...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1237378
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