The high cost of chemical pesticides and their negative impact on the environment prompted the search for natural pesticides from plants. The objective of our study was to control rice seed pathogenic fungi and bacteria using aqueous extract and essential oil from Tithonia diversifolia leaves. We obtained aqueous extract and essential oil, respectively, by maceration and hydrodistillation; the antimicrobial activities were determined in vitro on a solid medium by the food poisoning method. The secondary metabolites were determined by qualitative and quantitative assays; the chemical composition of the essential oil obtained from Titonia diversifolia was studied using gas-chromatography coupled with mass spectrometry. The results showed that phenols, tannins, flavonoids, alkaloids, terpenoids, sugars and saponins were present in the aqueous extract. The essential oil contained mainly hydrocarbonated, oxygenated monoterpenes, terpenoids and sesquiterpenes. α-terpineol (20.3%), eucalyptol (14.6%), camphor (14.3%) and α-pinene (13.5%) as the main compounds. Regarding the antimicrobial activity, all tested bacteria were sensitive to aqueous extract and essential oil. The activity of the aqueous extract on the tested fungi showed an inhibitory concentration 50 (IC50) of 50 mg/mL against Bipolaris oryzae and Fusarium moniliforme. The activity of the essential oil on bacteria and fungi showed MIC of 125 μg/mL (Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae) and MFC of 5000 μg/mL (Bipolaris oryzae and Fusarium moniliforme). These results allow us to consider Tithonia diversifolia as a potential source of natural biopesticides against rice seed-borne pathogens.

Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice / Dongmo, Albert Nanfack; Nguefack, Julienne; Dongmo, Joseph Blaise Lecagne; Fouelefack, François Romain; Azah, Rene Udom; Nkengfack, Ephrem Augustin; Stefani, Emilio. - In: JOURNAL OF PLANT DISEASES AND PROTECTION. - ISSN 1861-3829. - 128:3(2021), pp. 703-713. [10.1007/s41348-021-00439-w]

Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice.

Stefani, Emilio
Conceptualization
2021

Abstract

The high cost of chemical pesticides and their negative impact on the environment prompted the search for natural pesticides from plants. The objective of our study was to control rice seed pathogenic fungi and bacteria using aqueous extract and essential oil from Tithonia diversifolia leaves. We obtained aqueous extract and essential oil, respectively, by maceration and hydrodistillation; the antimicrobial activities were determined in vitro on a solid medium by the food poisoning method. The secondary metabolites were determined by qualitative and quantitative assays; the chemical composition of the essential oil obtained from Titonia diversifolia was studied using gas-chromatography coupled with mass spectrometry. The results showed that phenols, tannins, flavonoids, alkaloids, terpenoids, sugars and saponins were present in the aqueous extract. The essential oil contained mainly hydrocarbonated, oxygenated monoterpenes, terpenoids and sesquiterpenes. α-terpineol (20.3%), eucalyptol (14.6%), camphor (14.3%) and α-pinene (13.5%) as the main compounds. Regarding the antimicrobial activity, all tested bacteria were sensitive to aqueous extract and essential oil. The activity of the aqueous extract on the tested fungi showed an inhibitory concentration 50 (IC50) of 50 mg/mL against Bipolaris oryzae and Fusarium moniliforme. The activity of the essential oil on bacteria and fungi showed MIC of 125 μg/mL (Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae) and MFC of 5000 μg/mL (Bipolaris oryzae and Fusarium moniliforme). These results allow us to consider Tithonia diversifolia as a potential source of natural biopesticides against rice seed-borne pathogens.
2021
15-feb-2021
128
3
703
713
Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice / Dongmo, Albert Nanfack; Nguefack, Julienne; Dongmo, Joseph Blaise Lecagne; Fouelefack, François Romain; Azah, Rene Udom; Nkengfack, Ephrem Augustin; Stefani, Emilio. - In: JOURNAL OF PLANT DISEASES AND PROTECTION. - ISSN 1861-3829. - 128:3(2021), pp. 703-713. [10.1007/s41348-021-00439-w]
Dongmo, Albert Nanfack; Nguefack, Julienne; Dongmo, Joseph Blaise Lecagne; Fouelefack, François Romain; Azah, Rene Udom; Nkengfack, Ephrem Augustin; S...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1235354
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