The dissociation of the steroid from glucocorticoid-receptor-RNA complexes at 5 degrees C was evaluated in cytosolic and nuclear extracts prepared from Hela cells crosslinked in vivo with glutaraldehyde. Sample treatment with catalytically active RNase A prevented the dissociation of the steroid which was induced by sample dilution with buffer. Dilution of the extracts with boiled cytosol, instead, stabilized steroid-receptor interactions. We conclude that some heat-stable factor should be also associated with glucocorticoid-receptor-RNA complexes from crosslinked cells, stabilizing steroid-receptor interactions, and we propose that it could counteract the labilizing effect of RNA.

STABILIZATION OF GLUCOCORTICOID-RECEPTOR INTERACTIONS INVITRO BY REMOVAL OF RNA BOUND TO RECEPTOR COMPLEXES INVIVO / Rossini, Gian Paolo; Masci, G.. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 47:(1990), pp. 743-751.

STABILIZATION OF GLUCOCORTICOID-RECEPTOR INTERACTIONS INVITRO BY REMOVAL OF RNA BOUND TO RECEPTOR COMPLEXES INVIVO

ROSSINI, Gian Paolo;
1990

Abstract

The dissociation of the steroid from glucocorticoid-receptor-RNA complexes at 5 degrees C was evaluated in cytosolic and nuclear extracts prepared from Hela cells crosslinked in vivo with glutaraldehyde. Sample treatment with catalytically active RNase A prevented the dissociation of the steroid which was induced by sample dilution with buffer. Dilution of the extracts with boiled cytosol, instead, stabilized steroid-receptor interactions. We conclude that some heat-stable factor should be also associated with glucocorticoid-receptor-RNA complexes from crosslinked cells, stabilizing steroid-receptor interactions, and we propose that it could counteract the labilizing effect of RNA.
47
743
751
STABILIZATION OF GLUCOCORTICOID-RECEPTOR INTERACTIONS INVITRO BY REMOVAL OF RNA BOUND TO RECEPTOR COMPLEXES INVIVO / Rossini, Gian Paolo; Masci, G.. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 47:(1990), pp. 743-751.
Rossini, Gian Paolo; Masci, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/12353
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