Recently, we have found that platelet-derived growth factor (PDGF)-AB and transforming growth factor (TGF)-β1 induce shape changes in the immunocytes of the mollusc Mytilus galloprovincialis. Here we report that calphostin C and H-89, which specifically inhibit PKC and PKA, respectively, completely or in part inhibit the above effect. These data indicate the involvement of both pathways in growth factor-induced conformational changes in molluscan immunocytes. Furthermore, we show the presence of immunoreactive molecules for the Fos B, Jun D and Smad4 transcription factors. Stimulation by PDGF-AB or TGF-β1 up-regulate the intranuclear levels of these factors. Copyright (C) 1999 Elsevier Science Inc.
Platelet-derived growth factor and transforming growth factor-β induce shape changes in invertebrate immunocytes via multiple signalling pathways and provoke the expression of Fos-, Jun- and SMAD-family members / Ottaviani, E.; Malagoli, D.; Kletsas, D.. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY. - ISSN 1096-4959. - 122:4(1999), pp. 389-395. [10.1016/S0305-0491(99)00012-7]