The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two indipendent techniques: UVVis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
INTERACTIONS OF NUCLEIC-ACIDS WITH DISTAMYCINS - BINDING OF DST-3 TO D(CGTTTAAACG)2 AND D(CGTACGTACG)2 / Capobianco, Ml; Colonna, Fp; Forni, Arrigo; Garbesi, A; Iotti, S; Moretti, Irene; Samori, B; Tondelli, L.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 19:(1991), pp. 1695-1698.
INTERACTIONS OF NUCLEIC-ACIDS WITH DISTAMYCINS - BINDING OF DST-3 TO D(CGTTTAAACG)2 AND D(CGTACGTACG)2
FORNI, Arrigo;MORETTI, Irene;
1991
Abstract
The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two indipendent techniques: UVVis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
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