Fire blight is the most destructive bacterial disease of pome fruits: it is caused by Erwinia amylovora and its management remains cumbersome. Nowadays, treatments with several beneficial microorganisms can lead to an affective integrated pest management (IPM): nevertheless, their specific activity in enhancing plant defence mechanisms is only partially understood. An extensive 3-years study in a commercial pear orchard was carried out, to verify the efficacy of a commercial microbial consortium (Micosat F®, CCS Aosta srl, Italy) to control a fire blight outbreak. In parallel, we have used a dHPLC (denaturing High Performance Liquid Chromatography) and automated DNA fragment collection using the WAVE System to analyse and recover cDNA-AFLP fragments. This transcriptomic approach was applied to understand which complex transcriptional changes these microorganisms may have elicited in the plant-pathogen interaction. In the commercial orchard, the beneficial effects of microbial consortium were confirmed by a significant disease reduction starting from the first year of application, as compared with other treatments commonly used by orchardists in IPM strategies. Among the eighty-five transcriptderived fragments (TDFs) collected and identified through the cDNA-AFLP-dHPLC approach, fourteen were found involved in systemic acquired resistance (SAR) according to the available literature. The transcriptomic approach developed in this study has been a robust and user-friendly mRNA fingerprinting method for the identification of differentially expressed genes, where prior knowledge of specific gene sequences is not a prerequisite. Finally, we confirmed the activity of such the microbial consortium as resistance inducer.

ELICITATION OF RESISTANCE TO FIRE BLIGHT BY A MICROBIAL CONSORTIUM: SEARCH FOR AN EVIDENCE THROUGH A TRANSCRIPTOMIC APPROACH / Giovanardi, Davide; Catalano, Valentina; Verzelloni, Elena; Dondini, Luca; Stefani, Emilio. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 2239-7264. - 98:4 SUP(2016), pp. 46-47. ((Intervento presentato al convegno XXII Convegno Nazionale Società Italiana di Patologia Vegetale - SIPaV tenutosi a Roma nel 19-22 Settembre, 2016 [10.4454/jpp.v98i4sup.3779].

ELICITATION OF RESISTANCE TO FIRE BLIGHT BY A MICROBIAL CONSORTIUM: SEARCH FOR AN EVIDENCE THROUGH A TRANSCRIPTOMIC APPROACH

GIOVANARDI, Davide
Investigation
;
CATALANO Valentina
Methodology
;
VERZELLONI Elena
Methodology
;
STEFANI, Emilio
Conceptualization
2016

Abstract

Fire blight is the most destructive bacterial disease of pome fruits: it is caused by Erwinia amylovora and its management remains cumbersome. Nowadays, treatments with several beneficial microorganisms can lead to an affective integrated pest management (IPM): nevertheless, their specific activity in enhancing plant defence mechanisms is only partially understood. An extensive 3-years study in a commercial pear orchard was carried out, to verify the efficacy of a commercial microbial consortium (Micosat F®, CCS Aosta srl, Italy) to control a fire blight outbreak. In parallel, we have used a dHPLC (denaturing High Performance Liquid Chromatography) and automated DNA fragment collection using the WAVE System to analyse and recover cDNA-AFLP fragments. This transcriptomic approach was applied to understand which complex transcriptional changes these microorganisms may have elicited in the plant-pathogen interaction. In the commercial orchard, the beneficial effects of microbial consortium were confirmed by a significant disease reduction starting from the first year of application, as compared with other treatments commonly used by orchardists in IPM strategies. Among the eighty-five transcriptderived fragments (TDFs) collected and identified through the cDNA-AFLP-dHPLC approach, fourteen were found involved in systemic acquired resistance (SAR) according to the available literature. The transcriptomic approach developed in this study has been a robust and user-friendly mRNA fingerprinting method for the identification of differentially expressed genes, where prior knowledge of specific gene sequences is not a prerequisite. Finally, we confirmed the activity of such the microbial consortium as resistance inducer.
XXII Convegno Nazionale Società Italiana di Patologia Vegetale - SIPaV
Roma
19-22 Settembre, 2016
Giovanardi, Davide; Catalano, Valentina; Verzelloni, Elena; Dondini, Luca; Stefani, Emilio
ELICITATION OF RESISTANCE TO FIRE BLIGHT BY A MICROBIAL CONSORTIUM: SEARCH FOR AN EVIDENCE THROUGH A TRANSCRIPTOMIC APPROACH / Giovanardi, Davide; Catalano, Valentina; Verzelloni, Elena; Dondini, Luca; Stefani, Emilio. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 2239-7264. - 98:4 SUP(2016), pp. 46-47. ((Intervento presentato al convegno XXII Convegno Nazionale Società Italiana di Patologia Vegetale - SIPaV tenutosi a Roma nel 19-22 Settembre, 2016 [10.4454/jpp.v98i4sup.3779].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1205644
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