Malaria and other infectious diseases are major global public health problems, which need to be tackled using new technologies to cope with the lack of efficacious vaccines and emerging drug resistance. A recently developed anti-infectious concept based on nanomimics tested with Plasmodium falciparum is analyzed for the molecular parameters determining its applicability. Nanomimics - nanoscaled polymer-based mimics of host cell membranes - are designed with a reduced number of surface-exposed malaria parasite receptor molecules (heparin), resulting in less potent invasion inhibition as determined in antimalarial assays. In contrast, when shorter receptor molecules are used to form nanomimics, more molecules are needed to obtain nanomimic potency similar to nanomimics with longer receptor molecules. The interaction of heparin on nanomimics with the processed Plasmodium falciparum merozoite surface protein 1-42 (PfMSP142) have a high affinity, Kd = 12.1 ± 1.6 × 10-9 m, as measured by fluorescence cross-correlation spectroscopy (FCCS). This detailed characterization of nanomimics and their molecular variants are an important step towards defining and optimizing possible nanomimic therapies for infectious diseases. Modified nanomimics are tested for their invasion inhibition potency against malaria parasites. Polymer-based host cell membrane mimics (nanomimics) are designed with variable length and number of surface-exposed malaria receptor molecules (heparin). Furthmore, the interaction of nanomimics with a responsible parasite ligand is characterized in detail to define nanomimic activity.

Analysis of Molecular Parameters Determining the Antimalarial Activity of Polymer-Based Nanomimics / Najer, A.; Thamboo, S.; Duskey, J. T.; Palivan, C. G.; Beck, H. -P.; Meier, W.. - In: MACROMOLECULAR RAPID COMMUNICATIONS. - ISSN 1022-1336. - 36:21(2015), pp. 1923-1928. [10.1002/marc.201500267]

Analysis of Molecular Parameters Determining the Antimalarial Activity of Polymer-Based Nanomimics

Duskey J. T.;
2015

Abstract

Malaria and other infectious diseases are major global public health problems, which need to be tackled using new technologies to cope with the lack of efficacious vaccines and emerging drug resistance. A recently developed anti-infectious concept based on nanomimics tested with Plasmodium falciparum is analyzed for the molecular parameters determining its applicability. Nanomimics - nanoscaled polymer-based mimics of host cell membranes - are designed with a reduced number of surface-exposed malaria parasite receptor molecules (heparin), resulting in less potent invasion inhibition as determined in antimalarial assays. In contrast, when shorter receptor molecules are used to form nanomimics, more molecules are needed to obtain nanomimic potency similar to nanomimics with longer receptor molecules. The interaction of heparin on nanomimics with the processed Plasmodium falciparum merozoite surface protein 1-42 (PfMSP142) have a high affinity, Kd = 12.1 ± 1.6 × 10-9 m, as measured by fluorescence cross-correlation spectroscopy (FCCS). This detailed characterization of nanomimics and their molecular variants are an important step towards defining and optimizing possible nanomimic therapies for infectious diseases. Modified nanomimics are tested for their invasion inhibition potency against malaria parasites. Polymer-based host cell membrane mimics (nanomimics) are designed with variable length and number of surface-exposed malaria receptor molecules (heparin). Furthmore, the interaction of nanomimics with a responsible parasite ligand is characterized in detail to define nanomimic activity.
36
21
1923
1928
Analysis of Molecular Parameters Determining the Antimalarial Activity of Polymer-Based Nanomimics / Najer, A.; Thamboo, S.; Duskey, J. T.; Palivan, C. G.; Beck, H. -P.; Meier, W.. - In: MACROMOLECULAR RAPID COMMUNICATIONS. - ISSN 1022-1336. - 36:21(2015), pp. 1923-1928. [10.1002/marc.201500267]
Najer, A.; Thamboo, S.; Duskey, J. T.; Palivan, C. G.; Beck, H. -P.; Meier, W.
File in questo prodotto:
File Dimensione Formato  
marc.201500267.pdf

non disponibili

Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 791.47 kB
Formato Adobe PDF
791.47 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1204739
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact