The roughness of the implant surface and its chemistery and toporaphy were demonstrated to impact cells biological properties. A moderate roughness seems to allow better and faster adhesion and proliferation and to accelerate the biological processes. The different physical and chemical procedures used to obtain a certain microtopography modify the surface chemical composition and, in consequence of it, can affect the biological cell behavior. The ideal surface shuold be obtained through processes that do not modify the chemical composition maintaining a right roughness and microtopography. The aim of this investigation was to study the morphology, proliferation and pattern of distribution of human Dental Pulp Stem Cells (hDPSCs) after 5 days of growth on a laser micro-grooved implant surface (Laser-Lock, Bio Horizons IPH, Inc. Birmingham, AL 35244, USA) characterized by a micro geometric design obtained by computer-aided laser ablation without any chemical treatment. Data from SEM surface observation and XPS chemical analysis have highlighted the absence of contaminating chemical elements. The results from cell cultures indicated that the hDPSCs maintained a good morphology and well proliferated following the microgrooves direction.
Adhesion and proliferation of human dental pulp stem cells on a laser micro textured implant surface: An in vitro study / Conserva, Enrico; Consolo, Ugo; Bellini, Pierantonio. - In: ORAL HEALTH AND CARE. - ISSN 2399-9640. - 3:4(2018), pp. 1-6. [10.15761/OHC.1000154]
Adhesion and proliferation of human dental pulp stem cells on a laser micro textured implant surface: An in vitro study
CONSERVA, Enrico
;Consolo Ugo
;Bellini Pierantonio
2018
Abstract
The roughness of the implant surface and its chemistery and toporaphy were demonstrated to impact cells biological properties. A moderate roughness seems to allow better and faster adhesion and proliferation and to accelerate the biological processes. The different physical and chemical procedures used to obtain a certain microtopography modify the surface chemical composition and, in consequence of it, can affect the biological cell behavior. The ideal surface shuold be obtained through processes that do not modify the chemical composition maintaining a right roughness and microtopography. The aim of this investigation was to study the morphology, proliferation and pattern of distribution of human Dental Pulp Stem Cells (hDPSCs) after 5 days of growth on a laser micro-grooved implant surface (Laser-Lock, Bio Horizons IPH, Inc. Birmingham, AL 35244, USA) characterized by a micro geometric design obtained by computer-aided laser ablation without any chemical treatment. Data from SEM surface observation and XPS chemical analysis have highlighted the absence of contaminating chemical elements. The results from cell cultures indicated that the hDPSCs maintained a good morphology and well proliferated following the microgrooves direction.File | Dimensione | Formato | |
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