A simple and reliable reversed-phase high-performance liquid chromatographic method with coulo-metric detection is described for the quantitation of naltrexone and its metabolite, 6-beta-naltrexol, in plasma samples of healthy volunteers who received orally 50 mg of naltrexone. The analytes and the internal standard, naloxone, are extracted with an octadecyl solid-phase extraction column before chromatography. The mobile phase is 0.01 M potassium phosphate (pH 3)-acetonitrile (85:15, v/v) and it is pumped at 0.8 ml/min. The coulometric detector is formed by two electrodes set at +0.20 V and +0.70 V, with a palladium reference electrode. The limit of quantitation observed was 5 ng/ml for both naltrexone and 6-beta-naltrexol. This method can be used to investigate pharmacokinetic parameters of different pharmaceutical preparations of this opioid antagonist.
DETERMINATION OF NALTREXONE AND 6-BETA-NALTREXOL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION / Zuccaro, P; Altieri, I; Betto, P; Pacifici, R; Ricciarello, G; Pini, Luigi Alberto; Sternieri, E; Pichini, S.. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - STAMPA. - 567:(1991), pp. 485-490.
DETERMINATION OF NALTREXONE AND 6-BETA-NALTREXOL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION
PINI, Luigi Alberto;
1991
Abstract
A simple and reliable reversed-phase high-performance liquid chromatographic method with coulo-metric detection is described for the quantitation of naltrexone and its metabolite, 6-beta-naltrexol, in plasma samples of healthy volunteers who received orally 50 mg of naltrexone. The analytes and the internal standard, naloxone, are extracted with an octadecyl solid-phase extraction column before chromatography. The mobile phase is 0.01 M potassium phosphate (pH 3)-acetonitrile (85:15, v/v) and it is pumped at 0.8 ml/min. The coulometric detector is formed by two electrodes set at +0.20 V and +0.70 V, with a palladium reference electrode. The limit of quantitation observed was 5 ng/ml for both naltrexone and 6-beta-naltrexol. This method can be used to investigate pharmacokinetic parameters of different pharmaceutical preparations of this opioid antagonist.
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