By means of semiquantitative immunocytochemistry the distribution of galanin like-immunoreactivity (LI) in various diencephalic and subcortical telencephalic areas of young (3 months), adult (12 months) and aged (24 months) rats was evaluated. A significant decrease in galanin LI was observed in all the analysed areas of the aged rats when compared with the young ones. In the adult animals a decrease in the microdensitometrical values with respect to the young rats was present only in the periventricular hypothalamic nucleus. A disappearance of nerve cell bodies was also observed in the central amygdaloid nucleus of the aged animals. The present data show a widespread age-related decrease of galanin LI in discrete diencephalic and subcortical telencephalic areas and suggest an involvement of this peptide in the multiple impairments of neuroendocrine and autonomic functions related to aging.
AGE-RELATED DECREASE OF GALANIN IMMUNOREACTIVITY IN DISCRETE FOREBRAIN AREAS OF THE MALE-RAT ASSESSED BY SEMIQUANTITATIVE IMMUNOCYTOCHEMISTRY / Ferraguti, F; Zoli, Michele; Fuxe, K; Agnati, Luigi Francesco. - In: NEUROSCIENCE RESEARCH COMMUNICATIONS. - ISSN 0893-6609. - 9:(1991), pp. 83-89.