Aim: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model. Methods: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections. Histological examination was performed postinoculation. Results: EV-treated wounds exhibited a better restoration compared with the counterpart MSC treatment. ASC-EV-treated wounds were significantly better than BM-EVs (p = 0.036). Conclusion: EV topical inoculation provides restored architecture during cutaneous wound healing and represents a promising solution for regenerative medicine in children.
Extracellular vesicles derived from mesenchymal cells: Perspective treatment for cutaneous wound healing in pediatrics / Pelizzo, Gloria; Avanzini, Maria Antonietta; Icaro Cornaglia, Antonia; De Silvestri, Annalisa; Mantelli, Melissa; Travaglino, Paola; Croce, Stefania; Romano, Piero; Avolio, Luigi; Iacob, Giulio; Dominici, Massimo; Calcaterra, Valeria. - In: REGENERATIVE MEDICINE. - ISSN 1746-0751. - 13:4(2018), pp. 385-394. [10.2217/rme-2018-0001]
Extracellular vesicles derived from mesenchymal cells: Perspective treatment for cutaneous wound healing in pediatrics
Dominici, MassimoWriting – Review & Editing
;
2018
Abstract
Aim: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model. Methods: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections. Histological examination was performed postinoculation. Results: EV-treated wounds exhibited a better restoration compared with the counterpart MSC treatment. ASC-EV-treated wounds were significantly better than BM-EVs (p = 0.036). Conclusion: EV topical inoculation provides restored architecture during cutaneous wound healing and represents a promising solution for regenerative medicine in children.
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