EnglishAccumulating evidence suggests that metformin, used as an antidiabetic drug, possesses anti-cancer properties. Metformin reduced the incidence and growth of experimental tumors in vivo. In a randomized clinical trial among nondiabetic patients, metformin treatment significantly decreased the number of aberrant crypt foci compared to the untreated group with a follow-up of 1 month. In our study, HT29 cells were treated with graded concentrations of metformin, 10 mM/25 mM/50 mM for 24/48 h. We performed immunofluorescence experiments by means of confocal microscopy and western blot analysis to evaluate a panel of factors involved in apoptotic/autophagic processes and oxidative stress response. Moreover, HT29 cells treated with metformin were analyzed by a flow cytometry assay to detect the cell apoptotic rate. The results demonstrate that metformin exerts growth inhibitory effects on cultured HT29 cells by increasing both apoptosis and autophagy; moreover, it affects the survival of cultured cells inhibiting the transcriptional activation of Nuclear factor E2-related factor 2 (NRF-2) and nuclear factor-kappa B (NF-κB). The effects of metformin on HT29 cells were dose- and time-dependent. These results are very intriguing since metformin is emerging as a multi-faceted drug: It has a good safety profile and is associated with low cost and might be a promising candidate for the prevention or the treatment of colorectal cancer.
| Data di pubblicazione: | 2018 |
| Titolo: | Metformin induces apoptosis and alters cellular responses to oxidative stress in Ht29 colon cancer cells: Preliminary findings |
| Autore/i: | Sena, P; Mancini, S; Benincasa, M; Mariani, Francesco; Palumbo, C; Roncucci, L |
| Autore/i UNIMORE: | |
| Digital Object Identifier (DOI): | 10.3390/ijms19051478 |
| Rivista: | |
| Volume: | 19 |
| Fascicolo: | 5 |
| Pagina iniziale: | 1478 |
| Pagina finale: | 1494 |
| Codice identificativo ISI: | WOS:000435297000214 |
| Codice identificativo Scopus: | 2-s2.0-85047134422 |
| Codice identificativo Pubmed: | 29772687 |
| Citazione: | Metformin induces apoptosis and alters cellular responses to oxidative stress in Ht29 colon cancer cells: Preliminary findings / Sena, P; Mancini, S; Benincasa, M; Mariani, Francesco; Palumbo, C; Roncucci, L. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 19:5(2018), pp. 1478-1494. |
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