Background: Worldwide antibiotic abuse represents a huge burden, which can have a deep impact on pet and human health through nutrition and medicalization representing another way of antibiotic resistance transmission. Objective: We aimed our research to determine a possible complex formation between biological bone substrates, such as proteins, and Oxytetracycline (OTC), an approved antibiotic for use in zootechny, which might determine a toxic effect on K562 cells. Method: Cell viability and HPLC-ESI/QqToF assays were used to assess potential toxicity of bone extract derived from OTC-treated chickens according to standard withdrawal times and from untreated chickens at 24, 48 and 72h of incubation. Results: Cell culture medium with ground bone from chickens reared in the presence of OTC (OTC-CCM) resulted significantly cytotoxic at every incubation time regardless of the bone concentration while cell culture medium with ground bone from chickens reared without OTC (BIO-CCM) resulted significantly cytotoxic only after 72h of incubation. HPLC-ESI/QqToF assay ruled out the possible presence of OTC main derivatives possibly released by bone within culture medium until 1 μg/mL. Conclusion: The presence of a protein complex with OTC is able to exert a cytotoxic effect once released in the medium after 24-48h of incubation.

Oxytetracycline-Protein Complex: The Dark Side of Pet Food / DI CERBO, Alessandro; Scarano, Antonio; Pezzuto, Federica; Guidetti, Gianandrea; Canello, Sergio; Pinetti, Diego; Genovese, Filippo; Corsi, Lorenzo. - In: THE OPEN PUBLIC HEALTH JOURNAL. - ISSN 1874-9445. - 11:(2018), pp. 162-169. [10.2174/1874944501811010162]

Oxytetracycline-Protein Complex: The Dark Side of Pet Food

Alessandro Di Cerbo
;
Diego Pinetti;Filippo Genovese;Lorenzo Corsi
2018

Abstract

Background: Worldwide antibiotic abuse represents a huge burden, which can have a deep impact on pet and human health through nutrition and medicalization representing another way of antibiotic resistance transmission. Objective: We aimed our research to determine a possible complex formation between biological bone substrates, such as proteins, and Oxytetracycline (OTC), an approved antibiotic for use in zootechny, which might determine a toxic effect on K562 cells. Method: Cell viability and HPLC-ESI/QqToF assays were used to assess potential toxicity of bone extract derived from OTC-treated chickens according to standard withdrawal times and from untreated chickens at 24, 48 and 72h of incubation. Results: Cell culture medium with ground bone from chickens reared in the presence of OTC (OTC-CCM) resulted significantly cytotoxic at every incubation time regardless of the bone concentration while cell culture medium with ground bone from chickens reared without OTC (BIO-CCM) resulted significantly cytotoxic only after 72h of incubation. HPLC-ESI/QqToF assay ruled out the possible presence of OTC main derivatives possibly released by bone within culture medium until 1 μg/mL. Conclusion: The presence of a protein complex with OTC is able to exert a cytotoxic effect once released in the medium after 24-48h of incubation.
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Oxytetracycline-Protein Complex: The Dark Side of Pet Food / DI CERBO, Alessandro; Scarano, Antonio; Pezzuto, Federica; Guidetti, Gianandrea; Canello, Sergio; Pinetti, Diego; Genovese, Filippo; Corsi, Lorenzo. - In: THE OPEN PUBLIC HEALTH JOURNAL. - ISSN 1874-9445. - 11:(2018), pp. 162-169. [10.2174/1874944501811010162]
DI CERBO, Alessandro; Scarano, Antonio; Pezzuto, Federica; Guidetti, Gianandrea; Canello, Sergio; Pinetti, Diego; Genovese, Filippo; Corsi, Lorenzo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1161523
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