Both morphine and anandamide significantly stimulated cultured endothelial intracellular calcium level increases in a concentration-dependent manner in cells pre-loaded with fura 2/AM. Morphine is more potent than anandamide (approximately 275 vs. 135 nM [Ca]i), and the [Ca]i for both ligands was blocked by prior exposure of the cells to their respective receptor antagonist, i.e., naloxone and SR 171416A. Various opioid peptides did not exhibit this ability, indicating a morphine-mu3-mediated process. In comparing the sequence of events concerning morphine's and anandamide's action in stimulating both [Ca]i and nitric oxide production in endothelial cells, we found that the first event precedes the second by 40+/-8 sec. The opiate and cannabinoid stimulation of [Ca]i was attenuated in cells leeched of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity.

Morphine and anandamide stimulate intracellular calcium transients in human arterial endothelial cells: coupling to nitric oxide release / Fimiani, C; Mattocks, D; Cavani, F; Salzet, M; Deutsch, D G; Pryor, S; Bilfinger, T V; Stefano, G B. - In: CELLULAR SIGNALLING. - ISSN 0898-6568. - 11:3(1999), pp. 189-193.

Morphine and anandamide stimulate intracellular calcium transients in human arterial endothelial cells: coupling to nitric oxide release

Cavani, F
Methodology
;
1999

Abstract

Both morphine and anandamide significantly stimulated cultured endothelial intracellular calcium level increases in a concentration-dependent manner in cells pre-loaded with fura 2/AM. Morphine is more potent than anandamide (approximately 275 vs. 135 nM [Ca]i), and the [Ca]i for both ligands was blocked by prior exposure of the cells to their respective receptor antagonist, i.e., naloxone and SR 171416A. Various opioid peptides did not exhibit this ability, indicating a morphine-mu3-mediated process. In comparing the sequence of events concerning morphine's and anandamide's action in stimulating both [Ca]i and nitric oxide production in endothelial cells, we found that the first event precedes the second by 40+/-8 sec. The opiate and cannabinoid stimulation of [Ca]i was attenuated in cells leeched of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity.
1999
11
3
189
193
Morphine and anandamide stimulate intracellular calcium transients in human arterial endothelial cells: coupling to nitric oxide release / Fimiani, C; Mattocks, D; Cavani, F; Salzet, M; Deutsch, D G; Pryor, S; Bilfinger, T V; Stefano, G B. - In: CELLULAR SIGNALLING. - ISSN 0898-6568. - 11:3(1999), pp. 189-193.
Fimiani, C; Mattocks, D; Cavani, F; Salzet, M; Deutsch, D G; Pryor, S; Bilfinger, T V; Stefano, G B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1155919
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