OBJECTIVES: GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis. METHODS: Patients subjected to temporal artery biopsies (TABs) naïve from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated. RESULTS: Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor. CONCLUSION: IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.
Increased expression of interleukin-22 in patients with giant cell arteritis / Zerbini, Alessandro; Muratore, Francesco; Boiardi, Luigi; Ciccia, Francesco; Bonacini, Martina; Belloni, Lucia; Cavazza, Alberto; Cimino, Luca; Moramarco, Antonio; Alessandro, Riccardo; Rizzo, Aroldo; Parmeggiani, Maria; Salvarani, Carlo; Croci, Stefania. - In: RHEUMATOLOGY. - ISSN 1462-0332. - 57:1(2018), pp. 64-72.
Data di pubblicazione: | 2018 |
Titolo: | Increased expression of interleukin-22 in patients with giant cell arteritis |
Autore/i: | Zerbini, Alessandro; Muratore, Francesco; Boiardi, Luigi; Ciccia, Francesco; Bonacini, Martina; Belloni, Lucia; Cavazza, Alberto; Cimino, Luca; Moramarco, Antonio; Alessandro, Riccardo; Rizzo, Aroldo; Parmeggiani, Maria; Salvarani, Carlo; Croci, Stefania |
Autore/i UNIMORE: | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1093/rheumatology/kex334 |
Rivista: | |
Volume: | 57 |
Fascicolo: | 1 |
Pagina iniziale: | 64 |
Pagina finale: | 72 |
Codice identificativo ISI: | WOS:000419623800018 |
Codice identificativo Scopus: | 2-s2.0-85039070808 |
Codice identificativo Pubmed: | 28968695 |
Citazione: | Increased expression of interleukin-22 in patients with giant cell arteritis / Zerbini, Alessandro; Muratore, Francesco; Boiardi, Luigi; Ciccia, Francesco; Bonacini, Martina; Belloni, Lucia; Cavazza, Alberto; Cimino, Luca; Moramarco, Antonio; Alessandro, Riccardo; Rizzo, Aroldo; Parmeggiani, Maria; Salvarani, Carlo; Croci, Stefania. - In: RHEUMATOLOGY. - ISSN 1462-0332. - 57:1(2018), pp. 64-72. |
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