A high-performance thin-layer chromatographic method is described for the investigation of the methylation rates of uracil, 5-fluoro-, 5-chloro-, 5-bromo-, 5-methyl- and 5-nitrouracil and their N1- and N3-methyl derivatives. The method allowed the amounts of compounds obtained, the time required to complete the reaction and therefore the reactivity of the uracil derivatives to be determined. The N1- and N3-methyl and N1,3-dimethyl derivatives were synthesized and separated by droplet countercurrent chromatography and/or medium-pressure liquid chromatography and their R(F) values were determined in different solvent systems by thin-layer chromatography.
DETERMINATION OF THE REACTIVITY OF URACIL DERIVATIVES WITH RESPECT TO METHYL-IODIDE BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC DENSITOMETRY / Vampa, G; Benvenuti, Stefania; Pecorari, P.. - In: JOURNAL OF CHROMATOGRAPHY. - ISSN 0021-9673. - STAMPA. - 604:(1992), pp. 261-265.