Nowadays techniques for sensitive non-invasive, real-time monitoring of cell differentiation and maturation are highly demanded. In light of this, the development of electrochemical printed sensors impedance-based could represent a promising tool. In the present work, we developed 2D ink-jet printed sensors for myoblasts adhesion monitoring, using carbon-based ink on a substrate consisting in non-woven electrospun mats made in crosslinked poly(ε-caprolactone) (PCL). First of all, sensors printability was optimized and the biocompatibility tested. In order to determine the possibility to employ the prepared systems as scaffolds for dynamic cellular cultures, the mechanical response of the PCL scaffold was evaluated through the application of cyclic deformation tests. After that, electrical characterization of ink and substrate was performed, followed by electrochemical impedance-based measurements to evaluate myoblasts adhesion. Biocompatibility assessment showed good results for both carbon and PCL. Mechanical tests findings suggested that a training of 50 cycles and a proper value of strain should be applied before the cell seeding, in order to ensure a subsequent controlled strain amplitude. The sensorized scaffold allowed us to correlate cell adhesion with an increase of impedance module, in agreement with biocompatibility testing. Thus, this first preliminary testing suggested that this non-invasive impedance spectroscopy-based measurement system can be used for sensitive monitoring of cells adhesion, in static and moreover, as suggested from mechanical characterization, in dynamic conditions.

Carbon on poly(epsilon-caprolactone) (PCL) ink-jet printed sensor for monitoring cell cultures of myoblasts / Marziano, M.; Tonello, S.; Serzanti, M.; Borghetti, M.; Lopomo, N. F.; Serpelloni, M.; Pandini, S.; Merlettini, A.; Gualandi, C.; Focarete, M. L.; Messori, M.; Toselli, M.; Uberti, D.; Memo, M.; Dell’Era, P.; Sardini, E.. - 65:(2018), pp. 783-786. ((Intervento presentato al convegno Joint Conference of the European Medical and Biological Engineering Conference, EMBEC 2017 and Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2107 tenutosi a Tampere, Finland nel 2017 [10.1007/978-981-10-5122-7_196].

Carbon on poly(epsilon-caprolactone) (PCL) ink-jet printed sensor for monitoring cell cultures of myoblasts

Messori, M.;
2018

Abstract

Nowadays techniques for sensitive non-invasive, real-time monitoring of cell differentiation and maturation are highly demanded. In light of this, the development of electrochemical printed sensors impedance-based could represent a promising tool. In the present work, we developed 2D ink-jet printed sensors for myoblasts adhesion monitoring, using carbon-based ink on a substrate consisting in non-woven electrospun mats made in crosslinked poly(ε-caprolactone) (PCL). First of all, sensors printability was optimized and the biocompatibility tested. In order to determine the possibility to employ the prepared systems as scaffolds for dynamic cellular cultures, the mechanical response of the PCL scaffold was evaluated through the application of cyclic deformation tests. After that, electrical characterization of ink and substrate was performed, followed by electrochemical impedance-based measurements to evaluate myoblasts adhesion. Biocompatibility assessment showed good results for both carbon and PCL. Mechanical tests findings suggested that a training of 50 cycles and a proper value of strain should be applied before the cell seeding, in order to ensure a subsequent controlled strain amplitude. The sensorized scaffold allowed us to correlate cell adhesion with an increase of impedance module, in agreement with biocompatibility testing. Thus, this first preliminary testing suggested that this non-invasive impedance spectroscopy-based measurement system can be used for sensitive monitoring of cells adhesion, in static and moreover, as suggested from mechanical characterization, in dynamic conditions.
13-giu-2017
Joint Conference of the European Medical and Biological Engineering Conference, EMBEC 2017 and Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2107
Tampere, Finland
2017
65
783
786
Marziano, M.; Tonello, S.; Serzanti, M.; Borghetti, M.; Lopomo, N. F.; Serpelloni, M.; Pandini, S.; Merlettini, A.; Gualandi, C.; Focarete, M. L.; Messori, M.; Toselli, M.; Uberti, D.; Memo, M.; Dell’Era, P.; Sardini, E.
Carbon on poly(epsilon-caprolactone) (PCL) ink-jet printed sensor for monitoring cell cultures of myoblasts / Marziano, M.; Tonello, S.; Serzanti, M.; Borghetti, M.; Lopomo, N. F.; Serpelloni, M.; Pandini, S.; Merlettini, A.; Gualandi, C.; Focarete, M. L.; Messori, M.; Toselli, M.; Uberti, D.; Memo, M.; Dell’Era, P.; Sardini, E.. - 65:(2018), pp. 783-786. ((Intervento presentato al convegno Joint Conference of the European Medical and Biological Engineering Conference, EMBEC 2017 and Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2107 tenutosi a Tampere, Finland nel 2017 [10.1007/978-981-10-5122-7_196].
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1151016
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 0
social impact