When HeLa cells were incubated with tritiated dexamethasone mesylate at 2-degrees-C, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cytosolic and nuclear extracts revealed the presence of two monomeric receptor complex forms with estimated molecular masses of about 98 and 87 kDa. If cells were subjected to crosslinking with glutaraldehyde, a third form consisting of a 250 kDa oligomer was also detected. When HeLa cells were treated with dexamethasone mesylate at 37-degrees-C, and were subjected to crosslinking, electrophoresis of cytosolic extracts revealed that the content of 250 kDa oligomeric glucocorticoid-receptor complexes was drastically reduced, whereas their levels in nuclear extracts were not appreciably altered.
DETECTION OF GLUCOCORTICOID-RECEPTOR COMPLEX OLIGOMERS IN NUCLEAR EXTRACTS FROM CELLS EXPOSED TO HORMONE AT PHYSIOLOGICAL TEMPERATURE / Rossini, Gian Paolo; Malaguti, C.. - In: LIFE SCIENCES. - ISSN 0024-3205. - STAMPA. - 51:(1992), pp. 1517-1525.
DETECTION OF GLUCOCORTICOID-RECEPTOR COMPLEX OLIGOMERS IN NUCLEAR EXTRACTS FROM CELLS EXPOSED TO HORMONE AT PHYSIOLOGICAL TEMPERATURE
ROSSINI, Gian Paolo;
1992
Abstract
When HeLa cells were incubated with tritiated dexamethasone mesylate at 2-degrees-C, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cytosolic and nuclear extracts revealed the presence of two monomeric receptor complex forms with estimated molecular masses of about 98 and 87 kDa. If cells were subjected to crosslinking with glutaraldehyde, a third form consisting of a 250 kDa oligomer was also detected. When HeLa cells were treated with dexamethasone mesylate at 37-degrees-C, and were subjected to crosslinking, electrophoresis of cytosolic extracts revealed that the content of 250 kDa oligomeric glucocorticoid-receptor complexes was drastically reduced, whereas their levels in nuclear extracts were not appreciably altered.
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